Discovery, Isolation, and Identification of Antimalarials from Fungal Endophytes

We examined fungal endophytes for their ability to inhibit bacterial, fungal and protozoal pathogens. Once antimalarial activity was discovered and confirmed, we sought to isolate and identify the active constituent(s) of our extracts. Bioassay-guided fractionation techniques were used to isolate our active compound, the structure of which was elucidated using <super>1</super>H- and <super>13</super>C-NMR. 2D-NMR and LC-MS analyses helped confirm that our endophytes were indeed producing artemisinin. Growth versus production studies were conducted to optimize our growth and extraction conditions while several other variables, like temperature and culture medium, were evaluated in an effort to improve our yields. Artemisinin production under both light and dark conditions was evaluated to determine if our production was the result of enzyme activity. In addition, labeling studies were conducted using <super>13</super>C-labeled glucose.

Name thesis:degree_name

Ph.D. in Pharmaceutical Sciences

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biomolecular Sciences

Year dc:date.available

2012

Author dc:creator

• Lee, Andrekeus

Contributors dc:contributor

• John Williamson
• Mark Hamann
• Mitchell A. Avery

• Antimalarials
• Endophytes
• Malaria
• Pharmacy and Pharmaceutical Sciences

Repository record dc:identifier

https://egrove.olemiss.edu/etd/1449

OAI identifier oai:identifier

oai:egrove.olemiss.edu:etd-2448