{"id":{"repo_id":"mississippi","oai_identifier":"oai:egrove.olemiss.edu:etd-2448"},"canonical_url":"https://search.dev.ndltd.org/etd/mississippi/oai:egrove.olemiss.edu:etd-2448","repository":{"repo_id":"mississippi","name":"University of Mississippi","base_url":"https://egrove.olemiss.edu/do/oai/"},"display":{"title":"Discovery, Isolation, and Identification of Antimalarials from Fungal Endophytes","abstract":"We examined fungal endophytes for their ability to inhibit bacterial, fungal and protozoal pathogens. Once antimalarial activity was discovered and confirmed, we sought to isolate and identify the active constituent(s) of our extracts. Bioassay-guided fractionation techniques were used to isolate our active compound, the structure of which was elucidated using <super>1</super>H- and <super>13</super>C-NMR. 2D-NMR and LC-MS analyses helped confirm that our endophytes were indeed producing artemisinin. Growth versus production studies were conducted to optimize our growth and extraction conditions while several other variables, like temperature and culture medium, were evaluated in an effort to improve our yields. Artemisinin production under both light and dark conditions was evaluated to determine if our production was the result of enzyme activity. In addition, labeling studies were conducted using <super>13</super>C-labeled glucose.","abstract_html":"We examined fungal endophytes for their ability to inhibit bacterial, fungal and protozoal pathogens. Once antimalarial activity was discovered and confirmed, we sought to isolate and identify the active constituent(s) of our extracts. Bioassay-guided fractionation techniques were used to isolate our active compound, the structure of which was elucidated using &lt;super&gt;1&lt;/super&gt;H- and &lt;super&gt;13&lt;/super&gt;C-NMR. 2D-NMR and LC-MS analyses helped confirm that our endophytes were indeed producing artemisinin. Growth versus production studies were conducted to optimize our growth and extraction conditions while several other variables, like temperature and culture medium, were evaluated in an effort to improve our yields. Artemisinin production under both light and dark conditions was evaluated to determine if our production was the result of enzyme activity. In addition, labeling studies were conducted using &lt;super&gt;13&lt;/super&gt;C-labeled glucose.","abstract_has_math":false,"creators":["Lee, Andrekeus"],"institution":null,"degree_name":"Ph.D. in Pharmaceutical Sciences","degree_level":"Dissertation","degree_discipline":"Biomolecular Sciences","degree_department":null,"school":null,"contributors":["John Williamson","Mark Hamann","Mitchell A. Avery"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2012,"date_issued":"2012-01-01T08:00:00Z","date_published":"2012-01-01T08:00:00Z","updated_at":"2026-07-24T03:06:53Z","subjects":["Antimalarials","Endophytes","Malaria","Pharmacy and Pharmaceutical Sciences"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://egrove.olemiss.edu/etd/1449","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["John Williamson","Mark Hamann","Mitchell A. 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Once antimalarial activity was discovered and confirmed, we sought to isolate and identify the active constituent(s) of our extracts. Bioassay-guided fractionation techniques were used to isolate our active compound, the structure of which was elucidated using <super>1</super>H- and <super>13</super>C-NMR. 2D-NMR and LC-MS analyses helped confirm that our endophytes were indeed producing artemisinin. Growth versus production studies were conducted to optimize our growth and extraction conditions while several other variables, like temperature and culture medium, were evaluated in an effort to improve our yields. Artemisinin production under both light and dark conditions was evaluated to determine if our production was the result of enzyme activity. In addition, labeling studies were conducted using <super>13</super>C-labeled glucose."]},{"key":"dc:title","label":"Title","values":["Discovery, Isolation, and Identification of Antimalarials from Fungal Endophytes"]}]}],"canonical_facts":{"dc:contributor":["John Williamson","Mark Hamann","Mitchell A. Avery"],"dc:creator":["Lee, Andrekeus"],"dc:date.available":["2020-01-23T08:00:00Z"],"dc:description.abstract":["We examined fungal endophytes for their ability to inhibit bacterial, fungal and protozoal pathogens. Once antimalarial activity was discovered and confirmed, we sought to isolate and identify the active constituent(s) of our extracts. Bioassay-guided fractionation techniques were used to isolate our active compound, the structure of which was elucidated using <super>1</super>H- and <super>13</super>C-NMR. 2D-NMR and LC-MS analyses helped confirm that our endophytes were indeed producing artemisinin. Growth versus production studies were conducted to optimize our growth and extraction conditions while several other variables, like temperature and culture medium, were evaluated in an effort to improve our yields. Artemisinin production under both light and dark conditions was evaluated to determine if our production was the result of enzyme activity. In addition, labeling studies were conducted using <super>13</super>C-labeled glucose."],"dc:identifier":["https://egrove.olemiss.edu/etd/1449"],"dc:subject":["Antimalarials","Endophytes","Malaria","Pharmacy and Pharmaceutical Sciences"],"dc:title":["Discovery, Isolation, and Identification of Antimalarials from Fungal Endophytes"],"thesis:degree_discipline":["Biomolecular Sciences"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D. in Pharmaceutical Sciences"]},"updated_at":"2026-07-24T03:06:53Z"}