Effects of Inbreeding on Endangered Red Wolves (Canis rufus)

Inbreeding depression, the reduction in offspring fitness caused by mating among close relatives, is widespread in small populations and a major concern in conservation biology because it can affect population persistence. The negative effects of inbreeding results in the evolution of inbreeding avoidance behaviors; within small populations, such behaviors may encourage individuals to select mates outside of their respective species. Mate choice may also be facilitated by variation at major histocompatibility complex (MHC) genes, a gene group critical for immune response and disease resistance. Given broad impacts of inbreeding and MHC variation on fitness and behavior, evaluating their effects is an important component of wildlife management. My dissertation research examined how inbreeding and immunogenetic variation influenced fitness, disease susceptibility, and mating behavior in endangered wild red wolves (Canis rufus). I also evaluated mitochondrial DNA from ancient canid bones to inform an ongoing debate regarding the species status of red wolves. I found evidence for an ancient red wolf origin which supports contemporary red wolf management practices (Chapter 2). Although these analyses were not directly related to inbreeding, clarifying red wolf taxonomic status is vital for effective species conservation. With regard to inbreeding depression, I found that red wolves were extremely inbred but their fitness was not associated with inbreeding. However, more inbred wolves tended to be smaller, which may have an indirect effect on reproductive success (Chapter 3). Next, I evaluated how immunogenetic variation influenced disease susceptibility by collecting baseline disease prevalence in red wolves and sympatric coyotes (Canis latrans), and sequencing MHC and toll-like receptor (TLR) genes. Coyotes harbored more parasite species then wolves and may act as disease reservoirs for red wolves (Chapter 4). Red wolves had lower immune gene variation then coyotes; variation may have been maintained through positive selection at MHC genes (Chapter 5). There were also several TLR haplotypes which were correlated with disease susceptibility. Finally, I evaluated red wolves’ mate choice (Chapter 6). I found little evidence for pedigree kinship avoidance but red wolves may avoid mates with more similar MHC alleles. This could contribute to hybridization with coyotes to avoid MHC-similar mates.

Name thesis:degree_name

Doctor of Philosophy (PhD)

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Environmental Sciences

Grantor

Renewable Natural Resources

Year dc:date.available

2015

Author dc:creator

• Brzeski, Kristin E.

• ancient DNA
• toll-like receptors
• MHC
• mate choice
• hybridization
• inbreeding depression
• Canis
• coyote

Statement dc:rights

• withheld
• Secure the entire work for patent and/or proprietary purposes for a period of one year. Student has submitted appropriate documentation which states: During this period the copyright owner also agrees not to exercise her/his ownership rights, including public use in works, without prior authorization from LSU. At the end of the one year period, either we or LSU may request an automatic extension for one additional year. At the end of the one year secure period (or its extension, if such is requested), the work will be released for access worldwide.

Identifier

etd-11092015-145821
https://repository.lsu.edu/gradschool_dissertations/794

OAI identifier oai:identifier

oai:repository.lsu.edu:gradschool_dissertations-1793