Role of Membrane-Associated Components of MYCOPLASMA ARTHRITIDIS on the Biosynthesis of Humoral Antibodies and Arthritogenic Properties in Experimental Animals

<p>The virulence of animal-passaged Mycoplasma arthritidis (158) was demonstrated to be enhanced in rats by growth in oleic acid-enriched broth. The virulence of a laboratory-maintained culture of the same strain was unaffected by similar growth conditions. No differences either in the immune response as measured by complement-fixing (CF) antibodies or in the histogenesis of the disease were detected when rats were injected with animal-passaged M. arthritidis grown either in the presence or in the absence of oleic acid. All tests for metabolism-inhibiting (MI) antibodies in the rat were negative.</p> <p>A Mycoplasma-associated immunosuppression of the primary and of the secondary hemagglutinin (HA) response to a common Gram negative antigen (CA-) of Escherichia coli (014) was demonstrated in the rabbit when pre-incubated mixtures of CA(-) and M. arthritidis membranes were injected iv. A similar immunosuppression was demonstrated only for the secondary HA response to a common Gram positive antigen (CA+) of Staphylococcus aureus when pre-incubated mixtures of CA(+) and M. arthritidis membranes were employed as immunizing materials. Mixtures containing CA(+) appeared to interfere with MI antibody production in the rabbit.</p> <p>The findings suggest 1) that oleic acid may play a role in the virulence of animal-passaged M. arthritidis in the rat# 2) that MI antibody synthesis may be subject to competitive inhibition by (CA+) in the rabbit, and 3) that the immunosuppressive effect of M. arthritidis is associated with membrane components and is not limited to the host in which arthritogenic properties are manifested.</p>

Name thesis:degree_name

Doctor of Philosophy (PhD)

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Microbiology

Year

1973

Author dc:creator

• Bergquist, Lois M.

Contributors dc:contributor

• Ben H. S. Lau
• Charles E. Winter
• Robert L. Nutter
• Dick H. Koobs
• Walter E. Roop

• Biochemistry
• Microbiology
• Pathology
• Mycoplasmatales; Arthritis -- etiology; Antibody Formation

Statement dc:rights

• This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Language dc:language

English

Repository record dc:identifier

https://scholarsrepository.llu.edu/etd/1305

OAI identifier oai:identifier

oai:scholarsrepository.llu.edu:etd-2097