The virulence of animal-passaged Mycoplasma arthritidis (158) was demonstrated to be enhanced in rats by growth in oleic acid-enriched broth. The virulence of a laboratory-maintained culture of the same strain was unaffected by similar growth conditions. No differences either in the immune response as measured by complement-fixing (CF) antibodies or in the histogenesis of the disease were detected when rats were injected with animal-passaged M. arthritidis grown either in the presence or in the absence of oleic acid. All tests for metabolism-inhibiting (MI) antibodies in the rat were negative.
A Mycoplasma-associated immunosuppression of the primary and of the secondary hemagglutinin (HA) response to a common Gram negative antigen (CA-) of Escherichia coli (014) was demonstrated in the rabbit when pre-incubated mixtures of CA(-) and M. arthritidis membranes were injected iv. A similar immunosuppression was demonstrated only for the secondary HA response to a common Gram positive antigen (CA+) of Staphylococcus aureus when pre-incubated mixtures of CA(+) and M. arthritidis membranes were employed as immunizing materials. Mixtures containing CA(+) appeared to interfere with MI antibody production in the rabbit.
The findings suggest 1) that oleic acid may play a role in the virulence of animal-passaged M. arthritidis in the rat# 2) that MI antibody synthesis may be subject to competitive inhibition by (CA+) in the rabbit, and 3) that the immunosuppressive effect of M. arthritidis is associated with membrane components and is not limited to the host in which arthritogenic properties are manifested.
","abstract_html":"<p>The virulence of animal-passaged Mycoplasma arthritidis (158) was demonstrated to be enhanced in rats by growth in oleic acid-enriched broth. The virulence of a laboratory-maintained culture of the same strain was unaffected by similar growth conditions. No differences either in the immune response as measured by complement-fixing (CF) antibodies or in the histogenesis of the disease were detected when rats were injected with animal-passaged M. arthritidis grown either in the presence or in the absence of oleic acid. All tests for metabolism-inhibiting (MI) antibodies in the rat were negative.</p> <p>A Mycoplasma-associated immunosuppression of the primary and of the secondary hemagglutinin (HA) response to a common Gram negative antigen (CA-) of Escherichia coli (014) was demonstrated in the rabbit when pre-incubated mixtures of CA(-) and M. arthritidis membranes were injected iv. A similar immunosuppression was demonstrated only for the secondary HA response to a common Gram positive antigen (CA+) of Staphylococcus aureus when pre-incubated mixtures of CA(+) and M. arthritidis membranes were employed as immunizing materials. Mixtures containing CA(+) appeared to interfere with MI antibody production in the rabbit.</p> <p>The findings suggest 1) that oleic acid may play a role in the virulence of animal-passaged M. arthritidis in the rat# 2) that MI antibody synthesis may be subject to competitive inhibition by (CA+) in the rabbit, and 3) that the immunosuppressive effect of M. arthritidis is associated with membrane components and is not limited to the host in which arthritogenic properties are manifested.</p>","abstract_has_math":false,"creators":["Bergquist, Lois M."],"institution":null,"degree_name":"Doctor of Philosophy (PhD)","degree_level":"Dissertation","degree_discipline":"Microbiology","degree_department":null,"school":null,"contributors":["Ben H. S. Lau","Charles E. Winter","Robert L. Nutter","Dick H. Koobs","Walter E. Roop"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1973,"date_issued":"1973-06-01T07:00:00Z","date_published":"1973-06-01T07:00:00Z","updated_at":"2026-07-24T02:53:37Z","subjects":["Biochemistry","Microbiology","Pathology","Mycoplasmatales; Arthritis -- etiology; Antibody Formation"],"languages":["English"],"rights":["This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://scholarsrepository.llu.edu/etd/1305","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Ben H. S. Lau","Charles E. Winter","Robert L. Nutter","Dick H. Koobs","Walter E. Roop"]},{"key":"dc:creator","label":"Author","values":["Bergquist, Lois M."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Doctor of Philosophy (PhD)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biochemistry","Microbiology","Pathology","Mycoplasmatales; Arthritis -- etiology; Antibody Formation"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]},{"key":"dc:rights","label":"Dc Rights","values":["This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://scholarsrepository.llu.edu/etd/1305"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The virulence of animal-passaged Mycoplasma arthritidis (158) was demonstrated to be enhanced in rats by growth in oleic acid-enriched broth. The virulence of a laboratory-maintained culture of the same strain was unaffected by similar growth conditions. No differences either in the immune response as measured by complement-fixing (CF) antibodies or in the histogenesis of the disease were detected when rats were injected with animal-passaged M. arthritidis grown either in the presence or in the absence of oleic acid. All tests for metabolism-inhibiting (MI) antibodies in the rat were negative.
A Mycoplasma-associated immunosuppression of the primary and of the secondary hemagglutinin (HA) response to a common Gram negative antigen (CA-) of Escherichia coli (014) was demonstrated in the rabbit when pre-incubated mixtures of CA(-) and M. arthritidis membranes were injected iv. A similar immunosuppression was demonstrated only for the secondary HA response to a common Gram positive antigen (CA+) of Staphylococcus aureus when pre-incubated mixtures of CA(+) and M. arthritidis membranes were employed as immunizing materials. Mixtures containing CA(+) appeared to interfere with MI antibody production in the rabbit.
The findings suggest 1) that oleic acid may play a role in the virulence of animal-passaged M. arthritidis in the rat# 2) that MI antibody synthesis may be subject to competitive inhibition by (CA+) in the rabbit, and 3) that the immunosuppressive effect of M. arthritidis is associated with membrane components and is not limited to the host in which arthritogenic properties are manifested.
"]},{"key":"dc:title","label":"Title","values":["Role of Membrane-Associated Components of MYCOPLASMA ARTHRITIDIS on the Biosynthesis of Humoral Antibodies and Arthritogenic Properties in Experimental Animals"]}]}],"canonical_facts":{"dc:contributor":["Ben H. S. Lau","Charles E. Winter","Robert L. Nutter","Dick H. Koobs","Walter E. Roop"],"dc:creator":["Bergquist, Lois M."],"dc:description.abstract":["The virulence of animal-passaged Mycoplasma arthritidis (158) was demonstrated to be enhanced in rats by growth in oleic acid-enriched broth. The virulence of a laboratory-maintained culture of the same strain was unaffected by similar growth conditions. No differences either in the immune response as measured by complement-fixing (CF) antibodies or in the histogenesis of the disease were detected when rats were injected with animal-passaged M. arthritidis grown either in the presence or in the absence of oleic acid. All tests for metabolism-inhibiting (MI) antibodies in the rat were negative.
A Mycoplasma-associated immunosuppression of the primary and of the secondary hemagglutinin (HA) response to a common Gram negative antigen (CA-) of Escherichia coli (014) was demonstrated in the rabbit when pre-incubated mixtures of CA(-) and M. arthritidis membranes were injected iv. A similar immunosuppression was demonstrated only for the secondary HA response to a common Gram positive antigen (CA+) of Staphylococcus aureus when pre-incubated mixtures of CA(+) and M. arthritidis membranes were employed as immunizing materials. Mixtures containing CA(+) appeared to interfere with MI antibody production in the rabbit.
The findings suggest 1) that oleic acid may play a role in the virulence of animal-passaged M. arthritidis in the rat# 2) that MI antibody synthesis may be subject to competitive inhibition by (CA+) in the rabbit, and 3) that the immunosuppressive effect of M. arthritidis is associated with membrane components and is not limited to the host in which arthritogenic properties are manifested.
"],"dc:identifier":["https://scholarsrepository.llu.edu/etd/1305"],"dc:language":["English"],"dc:rights":["This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights."],"dc:subject":["Biochemistry","Microbiology","Pathology","Mycoplasmatales; Arthritis -- etiology; Antibody Formation"],"dc:title":["Role of Membrane-Associated Components of MYCOPLASMA ARTHRITIDIS on the Biosynthesis of Humoral Antibodies and Arthritogenic Properties in Experimental Animals"],"thesis:degree_discipline":["Microbiology"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Doctor of Philosophy (PhD)"]},"updated_at":"2026-07-24T02:53:37Z"}