Anticarcinogenic, Antitumor, and Antifungal Properties of Allium Sativum (Garlic)

<p>In this study the antimutagenic/anticarcinogenic, immune enhancing, antitumor and antifungal effects of a crude extract and organosulfur compounds of garlic were determined. Diallyl sulfide (DAS), and ajoene each at 100 /xg/ml, and garlic extract (GE, 12.5 mg/ml) inhibited rat liver 9000 X g supernatant (S- 9) dependent mutagenesis in <em>Salmonella typhimurium</em> TA100 induced by either aflatoxin B1 (AFB^ or benzo[a]pyrene (B[a]P). The garlic compounds were shown to inhibit the binding of [³H]AFB₁ to calf thymus DNA and the formation of specific AFB_1-DNA adducts. These effects on mutagenesis and DNA binding correlated with an inhibition of the metabolism of [³H]AFB_1. Ajoene and DAS did not effect glutathione-S-transferase, an enzyme important to the conjugation of compounds to glutathione.</p> <p>GE was tested for its effects on immune function in a murine model by injecting C3H/He mice with <em>Candida albicans.</em> GE appeared to control <em>C. albicans</em> as exhibited in blood and kidney cultures. Peritoneal exudate cells obtained for chemiluminescent assays from garlic-treated mice exhibited an increased oxidative burst.</p> <p>The cytotoxic effects of diallyl disulfide (DADS, 5-200 /xg/ml), DAS (200 /ig/ml), dimethyl disulfide (DMDS, 5-400 /xg/ml) and allyl methyl sulfide (AMS, 5-400 /xg/ml), were determined with in vitro cultures of MBT-2, R3327, HL-60 and K562 tumor cells. DADS exhibited toxicity against all the tumor cell lines tested. DMDS was cytotoxic to K562 tumor cells and AMS was cytotoxic to MBT-2 cells.</p> <p>The effects of DADS, DAS, DMDS and AMS on the <em>in vitro</em> growth of C. albicans were investigated. Inhibitory concentrations mg/ml of DAS, DMDS, AMS and DADS were in the range of 0.625 - 1.250, 5 - 20, 10 - 20 and 156-625 /ig/ml respectively.</p> <p>The results of this study suggest that garlic compounds inhibited rat liver S-9 mediated AFB, metabolite binding to calf thymus DNA, AFBrDNA adduct formation and AFBrinduced mutagenesis in <em>S. typhimurium</em> TA100 by inhibition of microsomal mixed-function oxidase activity. Garlic compounds exhibited antifungal activity and enhanced phagocytic activity. In addition, garlic compounds were cytotoxic to several tumor cell lines.</p>

Name thesis:degree_name

Doctor of Philosophy (PhD)

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biology

Year

1991

Author dc:creator

• Tadi, Padma P.

Contributors dc:contributor

• Benjamin H. S. Lau
• Leonard R. Brand
• Robert W. Teel
• Clifford E. Herrmann
• James D. Kettering
• Elwood McCluskey

• Biology
• Food Microbiology
• Garlic -- analysis

Statement dc:rights

• This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Language dc:language

English

Repository record dc:identifier

https://scholarsrepository.llu.edu/etd/918

OAI identifier oai:identifier

oai:scholarsrepository.llu.edu:etd-1904