The Effects of Biological Response Modification and Diet on the Immune Respose of Tumor-bearing mice

<p>The effect that diet and other modifiers may have upon the immune system of BALB/c mice bearing tumor were studied, mice were injected s.c. with 10⁶herpes simplex virus Type 2- Adult male BALB/c transformed (H238) cells in the thigh causing a visible fibrosarcoma within 1-2 weeks. Mice were fed either a diet low in protein (milk) and low in fat (Diet 1), or another diet low in protein (wheat) and high in fat (Diet 8). Spleen cells were collected for assays of cytotoxic T-lynphocyte (CTL) and natural killer (NK) cell activity. At 3 weeks post-injection, a decrease in CTL activity against H238 tumor cells was observed but no change in NK activity against H238 or K562 targets was seen. Spleen cells were treated In vitro with mouse interferon, rat interleukin-2, 2-amino-5-bromo-6-methyl-4-pyrimidinone (ABMP), Prostaglandin E₁ (PGE₁), singly and in combination, for 2 hours prior to mixing with target cells. Treated spleen cells from Diet 1 tumor-bearing animals at 3-6 weeks post-injection had substantially increased CIL response while those from Diet 8 mice were only slightly enhanced. Interferon, interleukin-2 and prostaglandin E₁ most effective. NK activity remained essentially unchanged regardless were of diet group or modifying treatment.</p>

Name thesis:degree_name

Master of Arts (MA)

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Microbiology

Year

1984

Author dc:creator

• Lovell, David Grant

Contributors dc:contributor

• James D. Kettering
• Robert L. Nutter
• Daila S. Gridley
• George M. Lessard

• Microbiology
• Immune System; Neoplasms -- diet therapy; Mice, Inbred BALB C

Statement dc:rights

• This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Language dc:language

English

Repository record dc:identifier

https://scholarsrepository.llu.edu/etd/725

OAI identifier oai:identifier

oai:scholarsrepository.llu.edu:etd-1778