CD21 and CD24 Co-expression: A Translational Model between Mouse and Human

<p>Systemic Lupus Erythematosus and Rheumatoid Arthritis are B cell-mediated autoimmune diseases that afflict millions of people worldwide. B cell-targeted therapies for these diseases result in variable clinical outcomes. Thus, a need exists to better understand the dynamics of human B cell production and function. The mouse model has provided a foundation for understanding the mechanisms involved in human B cell development and autoimmune disease. However, differences in mouse and human B cells are not fully understood. Our work shows that the co-expression of CD21 and CD24, determined by 7-color flow cytometry, can be used to demarcate developmental subsets of B cells. A comparison of analogous B cell subsets in mice and humans showed that the B cell subsets distribution differs between the species suggesting differences exist in mechanisms that regulate and maintain the specific B cell. This work provides a foundation for understanding human B cell development using the mouse model.</p>

Name thesis:degree_name

Doctor of Philosophy (PhD)

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Basic Sciences

Year

2014

Author dc:creator

• Benitez, Abigail

Contributors dc:contributor

• Payne, Kimberly J.
• Casiano, Carlos A.
• Colburn, Keith K.
• Duerksen-Hughes, Penelope
• Gridley, Daila
• Soto-Wegner, Ubaldo

• Genetics and Genomics
• Life Sciences
• Medicine and Health Sciences
• Microbiology
• Molecular Genetics
• Antigens - Differentiation; Antigens - CD24; Receptors - Complement 3d; B-Lymphocyte Subset; B-Cell Activating Factor; Lymphoid Progenitor Cells; Hematopoietic Stem Cells; Immunoglobulin Light Chains; Common Variable Immunodeficiency; Species Specificity
• Systemic Lupus Erythematosus; Rheumatoid Arthritis; B cell-mediated autoimmune diseases; Mouse model; Human B Cell development;

Statement dc:rights

• This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Language dc:language

English

Repository record dc:identifier

https://scholarsrepository.llu.edu/etd/198

OAI identifier oai:identifier

oai:scholarsrepository.llu.edu:etd-1202