Semiquantitative und quantitative Charakterisierung der Zytokinantwort humaner Keratinozyten nach Stimulation mit Propionibacterium acnes und Koproporphyrin III

Background The inflammation in acne vulgaris is widely thought to be induced by an immunological reaction to Propionibacterium acnes. Objectives In the present study we examined the local host response mechanism of keratinocytes 3 and 6 h after stimulation with viable and heat- killed P. acnes. Methods The quantitative expression of cytokines was measured at the mRNA level by real time RT-PCR. Results The co-incubation of keratinocytes with active, but not heat- killed, P. acnes modulated an adequate cytokine response for IL-1beta, GM-CSF, and IL-8. High-performance liquid chromatography analysis of the in vivo porphyrin pattern secreted by P. acnes revealed a predominance of coproporphyrin III in inflammatory acne lesions. This same porphyrin fraction also modestly induced IL-8 expression by keratinocytes. Conclusions This cytokine pattern may favor a chemotactic response and implicates P. acnes and coproporphyrin III in the recruitment of leukocytes to the site of infection and in the development of inflammatory lesions.

Level thesis:degree_level

thesis.doctoral

Grantor dc:publisher

Ludwig-Maximilians-Universität

Year

2004

Author dc:creator

• Löwenstein, Melitta

Repository record source_url

https://edoc.ub.uni-muenchen.de/1792/

OAI identifier oai:identifier

oai:edoc.ub.uni-muenchen.de:1792