Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 100 for “"Real Time RT-PCR"”.
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Development of a single real-time RT-PCR method for the group-specific identification of African horsesickness virus and bluetongue virus
… that can result from outbreaks. Bluetongue, an arthropod-transmitted disease of wild and domestic ruminants, is caused by the bluetongue virus, which is the prototype species of the genus Orbivirus. Bluetongue is also a notifiable disease because it can spread very rapidly in naive populations of …
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Real Time RT-PCR for Direct Detection of Viable Mycobacterium Avium Subspecies paratuberculosis in Chron's Disease Patients and Association of Map Infection with Downregulation in Interferon-Gamma Receptor (INFG1) Gene in Crohn's Disease Patients
… of cell wall in infected patients. The advent of real time PCR has proven to be significant in infectious disease diagnostics. In this study, real time reverse transcriptase PCR (RT-PCR) assay based on targeting mRNA of the IS900 gene unique to MAP has been developed. All variables included in RNA …
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Etablierung einer Zellkultur von PDL-Fibroblasten aus parodontal erkranktem Zahnhalteapparat des Menschen
Diese Dissertation beschäftigt sich mit der Etablierung einer Zellkultur aus parodontal erkrankten PDL-Zellen unter Entwicklung einer spezifischen Antibiose zur Kultivierung und einer sich daran anschließenden vergleichenden Charakterisierung der Genexpressionsmuster von gesunden und erkrankten …
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Ανάπτυξη και κλινικές εφαρμογές μεθόδων μοριακής διαγνωστικής για την ανίχνευση κυκλοφορούντων καρκινικών κυττάρων στον καρκίνο του μαστού μέσω της έκφρασης της μαστοσφαιρίνης α
… The method involves: a) the use of nested PCR in peripheral blood of early breast cancer patients. All primers were in silico designed and evaluated in order to avoid false positive results due to genomic DNA contamination. The specificity of the primers used was evaluated in 30 healthy …
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Isolation and functional analysis of differentially expressed genes in human prostate cancer
Prostatakrebs ist der am häufigsten prognostizierte solide Tumor und die zweithäufigste Ursache für Krebstod bei Männern in den westlichen Ländern. Eine der Schlüsselaufgaben der Prostatakrebsforschung ist es, molekulare Marker zu entwickeln, die effektiv Progression und Malignität von …
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The Effect of Gonadotropin Releasing Hormone on Opsin Gene Expression and Spectral Sensitivity in Zebra Cichlid Fish (Metriaclima zebra).
… in spectral sensitivity of cichlids has been partially attributed to differences in opsin gene expression, with each species preferentially expressing a subset of seven possible genes. Hormones such as gonadotropin releasing hormone (GnRH) can mediate changes in gene expression and the presence …
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Vip And Host Immunity
<p>The purpose of the current dissertation was to examine how VIP regulates host immunity and corneal healing, specifically, its control of growth factors and TLR expression in the P. aeruginosa infected cornea. Firstly, VIP treatment increased growth factor expression (EGF, GHF, FGF and VEGF) in …
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Differential gene expression and immune regulatory mechanisms in parasite-resistant hair and susceptible wool sheep infected with the parasitic nematode, Haemonchus contortus
… the parasitic nematode Haemonchus contortus is a major animal health concern. Caribbean hair sheep are more resistant than conventional wool breeds to this blood-feeding, abomasal parasite. Our objective was to determine differences in the immune response associated with …
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A study on endocrine disrupters in the environment through the microarray technology
… described as xenoestrogens, is the major part of this debate. Humans beings are exposed to these substances from different environmental contaminations ranging from conscious intake of estrogenic substances, as in contraception or in hormone replace therapy (HRT), to unconscious exposure, …
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Structural and functional studies of VP9, a novel nonstructural protein from white spot syndrome virus
… by ORF WSV230, was identified for the first time, in the infected Penaeus monodon shrimp tissues, gill and stomach as a novel, nonstructural protein by western blot, mass spectrometry and immuno-electron microscopy. Real-time RT-PCR demonstrated that the transcription of VP9 started from the …
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Semiquantitative und quantitative Charakterisierung der Zytokinantwort humaner Keratinozyten nach Stimulation mit Propionibacterium acnes und Koproporphyrin III
… of cytokines was measured at the mRNA level by real time RT-PCR. Results The co-incubation of keratinocytes with active, but not heat- killed, P. acnes modulated an adequate cytokine response for IL-1beta, GM-CSF, and IL-8. High-performance liquid chromatography analysis of the in vivo porphyrin …
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Early divergent B cell differentiation during antibody responses
… Varying the antigen dose altered the proportion of B cells that entered the extra follicular or GC responses and the amount of class switch recombination (CSR). The expression of phenotypic markers and switched antibody protein in parallel with gene array analysis and single cell real time …
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Modulating activity of vancomycin and daptomycin on the expression of gene involved in reduced glycopeptide susceptibility in Staphylococcus aureus
… (Methicillin Resistant S.aureus-MRSA), ha portato all'insorgenza di ceppi con ridotta sensibilità a vancomicina noti come heterogeneous Vancomycin-Intermediate S.aureus (hVISA) e Vancomycin-Intermediate S.aureus (VISA). Le basi genetiche della ridotta sensibilità a vancomicina sono state …
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Screening for kinases regulating subcellular localization of clock proteins using human kinome library
… 9 D. Fluorescence microscopy 11 E. Quantitative real-time RT-PCR 12 F. Cell lysis 2 G. Western blotting 13 H. Immunoprecipitation (IP) and λphosphatase treatment 14 I. Fly genetics 15 J. Locomotor activity assays 16 Ⅲ. 결과 17 A. 인간 인산화 효소 라이브러리 구축 17 B. 인간 인산화 효소 스크리닝 20 C. VRK3 27 1. …
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An Assessment of the Molecular Basis of Toxin-induced Dilated Cardiomyopathy in an Avian Animal Model
… a marked decrease in the left ventricular shortening fraction. Pathological phenotype confirmed the dilated heart with extended cell necrosis. Two mutations, both in NADH dehydrogenase genes, were found to be associated with DCM. Real-time RT-PCR quantification indicated that mRNA expression …
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Immune function alterations in Asian elephants (Elephas maximus) infected with Mycobacterium species
Tuberculosis is an important health concern for Asian elephant (Elephas maximus) populations worldwide. Most infections are due to Mycobacterium tuberculosis, the cause of human tuberculosis, though mechanisms underlying elephant susceptibility are unknown. In humans and other studied species, …
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The Expression of T-Box (Tbx3) in the Bovine Mammary Gland
… ng/mL for 24 and 48 hours. As determined by real-time RT-PCR, we did not observe a change in Tbx3 expression in cells treated with GH (P ≥ 0.74). However, both IGF-I treatments increased Tbx3 expression (P ≤ 0.03). Surprisingly, expression of Tbx2 was not detectable in MEC cells; however it …
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Classification of Neuronal Nicotinic Acetylcholine Receptors in Rat CA1 Hippocampal Interneuron Subpopulations Defined by Calcium-Binding Protein mRNA Expression
… rat hippocampi was analyzed using quantitative real time RT-PCR. Eighty-seven percent of the interneurons examined expressed CaBP mRNA. Parvalbumin mRNA was detected in 64%, while calbindin and calretinin expression was detected in 26% and 40% of interneurons, respectively. CaBP expression was …
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Untersuchungen zur Physiologie des Essigsäurebakteriums Gluconobacter oxydans 621H
… und wächst optimal bei 25 bis 30 °C und pH-Werten von 5,5 bis 6,0. Aufgrund der Fähigkeit zur unvollständigen Oxidation eines weiten Spektrums organischer Substrate, insbesondere von Zuckern oder Alkoholen, ist G. oxydans aber auch von großer biotechnologischer Bedeutung. Für diese Oxidationen …
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Anti-inflammatory Effects and Biodistribution of Cerium Oxide Nanoparticles
Cerium oxide nanoparticles have the unique ability to accept and donate electrons, making them powerful antioxidants. Their redox nature is due to oxygen defects in the lattice structure, which are more abundant at the nanoscale. Reactive oxygen species (ROS) are pro-oxidants whose presence is …
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