Lethbridge, Alta. : Universtiy of Lethbridge, Department of Biological Sciences
Engineering dual-glycan responsive expression systems for tunable production of heterologous proteins in Bacteroides thetaiotaomicron
Abstract
dc:description.abstractGenetically engineering symbiotic bacteria remains an underexploited opportunity to improve host-health and create new classes of biological devices, such as diagnostics or intestinal delivery systems for therapeutics. Bacteroides thetaiotamicron (B. theta) is a Gram-negative intestinal anaerobe with potential for the capability to produce functional heterologous proteins within a host intestine. To improve the strength and regulatory fidelity of transgene expression in B. theta, I have developed platform expression strains with engineered regulatory proteins under control of promoter elements that respond to dextran and arabinogalactan, two chemically distinct glycans. In addition to single glycan induction, I have also developed a novel “dual-glycan” expression system that requires the addition of both dextran and arabinogalactan for induction. Additionally my engineered strains are compatible with a series of chromosomal integration and episomal vectors that improve the throughput of gene cloning, integration, and expression. Together this expression system provides a new collection of glycan-responsive tools to improve transgene expression in B. theta and provides the proof-of-concept for engineering more complex dual-glycan expression systems.
Degree
thesis:*- Grantor dc:publisher
- Lethbridge, Alta. : Universtiy of Lethbridge, Department of Biological Sciences
- Year dc:date.issued
- 2018
Author and committee
dc:creator, dc:contributor.*- Authors dc:creator
-
- Smith, Marshall B.
- University of Lethbridge. Faculty of Arts and Science
- Advisors dc:contributor.supervisor
-
- Abbott, D. Wade
- Selinger, L. Brent
Subjects
dc:subject × 11Rights
- Language dc:language.iso
- en_US
Identifiers
dc:identifier.*- Identifier
- hdl:10133/5416