Mechano-sensitivity of nuclear lamin proteins in endothelial cells

Atherosclerosis is a chronic disease that happens mostly in aged people, and recently studies have showed many similarities between Hutchinson Gilford Progeria Syndrome (HGPS) cells and aging cells, implicating dysfunctions of lamin A/C in aging process and atherosclerosis, as HGPS is caused by a mutated form of lamin A/C. Blood flow in arteries is generating shear stress that is mostly applied on endothelial cells that align along inner blood vessel wall. At the same time, endothelial cells are also under stretch by periodic arterial pulses. Considering the fact that atherosclerosis is prone to developing at arterial branches with disturbed shear and increased stretch, it is highly possible that laminar flow and proper stretch force are regulating endothelium to function appropriately. In this thesis, the investigation of what effects laminar flow or cyclic stretch can bring to endothelial cells was conducted, and examination of lamin A/C expression under mechanical forces were elaborated and incorporated with cell senescence. Results showed that laminar shear stress and stretch force can regulate lamin A/C expression in different patterns, which were impaired in senescent cells.

Author dc:creator

• Jiang, Yizhi

Advisor dc:contributor.advisor

• Ji, Julie Ying Hui

• lamin A/C
• shear stress
• stretch
• aging
• HGPS
• endothelial cells

Language dc:language.iso

en_US

DOI

10.7912/C2TG7C

Identifier URI

http://dx.doi.org/10.7912/C2/1353

OAI identifier oai:identifier

oai:scholarworks.indianapolis.iu.edu:1805/10913