Quantifying T cell Mediated Killing for Melanoma Immunotherapy

Adoptive cell therapy (ACT), based on the adoptive transfer of tumor-infiltrating lymphocytes (TILs) has well-recognized advantages such as (1) high specificity for target cells; (2) the ability to target even micrometastases; (3) the potential to proliferate in vivo within the host thus increasing both surveillance and destruction capabilities, and (4) the feasibility to treat late stage tumors refractory to all other treatment methods with clinical response rates of ~50%. Despite numerous improvements in the last decade, ACT treatments still result in a wide range of outcomes. Functional heterogeneity, at the single-cell level, of cells infused for ACT has not been routinely characterized and consequently their efficacy and persistence in vivo following ACT are unpredictable. By using a high-throughput single-cell methodology, we demonstrate here that our assay has been able to quantify and indentify sub-populations within a TILs sample based on their individual killing potentials when matched with different number of targets.

Name thesis:degree_name

Master of Science

Level thesis:degree_level

Masters

Discipline thesis:degree_discipline

Chemical Engineering

Grantor

University of Houston

Year dc:date.issued

2014

Author dc:creator

• Vu, Thai

Advisor dc:contributor.advisor

• Varadarajan, Navin

Committee members dc:contributor.committeemember

• Krishnamoorti, Ramanan
• Roysam, Badrinath
• Vekilov, Peter G.

• Quantitative
• Immunology

Statement dc:rights

• The author of this work is the copyright owner. UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. Further transmission, reproduction, or presentation of this work is prohibited except with permission of the author(s).

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/10657/1684

OAI identifier oai:identifier

oai:uh-ir.tdl.org:10657/1684