Η συμβολή της ραδιοανοσολογικής μεθόδου στη μελέτη της φαρμακοκινητικής της ιντερφερόνης σε ασθενείς με νεοπλάσματα

The use of interferon alpha is currently established for the treatment of certain neoplastic diseases and among them for the management of malignant melanoma. Considering the extremely high cost of this product and the existing relationship between the therapeutical results and the levels of the drug in the serum, the individualization of dose becomes a necessity. The pharmacokinetics of interferon alpha was studied in ten consecutive patients suffering from malignant melanoma who were hospitalized at the First Chemotherapy Clinic of "Theagenion" Cancer Institute. Two methods for identifying the levels of the substance in the serum were used: The inhibition of the enzyme 2’5 oligo (A) synthetase and the radioimmunoassay (RIA). The aim of this study was to check if the RIA, due to its simplicity and rapidness can be used as a routine substituting the well known and widely used for year’s enzymatic method. All the patients were included for the first time in an interferon protocol with 5 to 10 X10⁶ International Units of interferon alpha (Intron A), administered by intramuscular injection. Blood samples were drawn before and then in specific time intervals six, twelve and twenty four hours after the administration of the drug. A wide distribution of the values between the different patients was observed, even when they received the same dose. The coefficient of variance was excellent (R²=0.98) when the mean values of the estimations in the different time intervals were compared. Concerning the RIA, the observed by previous investigators luck of sensitivity in the low interferon levels was not evident in this study. As a conclusion it can be stated that RIA can be utilized for the estimation of the interferon's levels in the sera of the patients suffering from malignant melanoma who receive therapeutic doses of this medicine. The quite good sensitivity which is obvious either at the low titers, combined with the other unequivocal advantages such as the simplicity, rapidness and the specificity make the RIA the method of choice. Additionally it identifies only the interferon alpha and is not influenced by the other kinds of interferons. It is a method that can be used in any Nuclear Medicine Department with elementary instrumentation for in vitro exams and can provide results only in few hours after the withdraw of the blood sample. On the other hand, the clinician is interested mainly in the maximal level of the drug and when this is achieved, because these factors will specify the therapeutic result. These parameters can be easily and accurately be identified by RIA, because high titers as such are obviously among the limits of the method.

Grantor dc:publisher

Aristotle University Of Thessaloniki (AUTH)

Year dc:date

1990

Authors dc:creator

• Doumas, Argyrios
• Δούμας, Αργύριος

• Ιατρική και Επιστήμες Υγείας
• Κλινική Ιατρική
• Επιστήμες Υγείας
• Medical and Health Sciences
• Clinical Medicine
• Health Sciences

Language dc:language

gre

Identifier

10.12681/eadd/1336

OAI identifier oai:identifier

oai:10442/1336