ΚΑΘΑΡΙΣΜΟΣ ΚΑΙ ΜΕΛΕΤΗ ΙΔΙΟΤΗΤΩΝ ΜΙΑΣ Β-ΓΛΥΚΟΣΙΔΑΣΗΣ ΚΑΙ ΜΙΑΣ Β-ΓΑΛΑΚΤΟΣΙΔΑΣΗΣ ΑΠΟ ΣΠΕΡΜΑΤΑ ΚΡΙΘΑΡΙΟΥ. ΕΦΑΡΜΟΓΗ ΤΩΝ ΕΝΖΥΜΩΝ ΣΤΗΝ ΥΔΡΟΛΥΣΗ ...

TWO ENZYMES A B-GLUCOSIDASE AND A B-GALACTOSIDASE WERE ISOLATED AND CHARACTERIZED FROM BARLEY SEEDS. THE B-GLUCOSIDASE IS A SINGLE BASIC POLYPEPTIDE (PI>8,5) WITH A MR OF 53.000 ACTING OPTIMALLY AT PH 4.5-5.0. THE B-GALACTOSIDASE IS COMPOSED OF TWO SUBUNITS WITH A MR OF 42.000 AND 33.000 RESPECTIVELY, IS ACIDIC IN NATURE (PI<5.7) AND ACTS OPTIMALLY AT PH 4.0. BOTH ENZYMES CAN HYDROLYZE LACTOSE, IN PURE SOLUTION OR IN WHEY, THEY ARE GLYCOPROTEINS THEY EXHIBIT CHARGE HETEROGENEITY AND CAN CATALYZE TRANSGALACTOSYLATION REACTIONS. B-GLUCOSIDASE WAS IMMOBILIZED ON SEPHAROSE, ON RESIN (DUOLITE) AND ON POROUS GLASS BEADS WITH SIGNIFICANT CONSERVATION OF ITS ACTIVITY. THE SEPHAROSE IMMOBILIZED ENZYME ACQUIRED INCREASED THERMOSTABILITY.

Grantor dc:publisher

Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ)

Year dc:date

1990

Author dc:creator

• Σίμος, Γεώργιος

• B-GALACTOSIDASE
• B-GLUCOSIDASE
• BARLEY (HORDEUM VULGARE)
• IMMOBILIZATION OF B-GLUCOSIDASE
• LACTOSE HYDROLYSIS
• TRANSGALACTOSYLATION
• Β-ΓΑΛΑΚΤΟΣΙΔΑΣΗ
• Β-ΓΛΥΚΟΣΙΔΑΣΗ
• ΚΑΘΗΛΩΣΗ (ΑΚΙΝΗΤΟΠΟΙΗΣΗ) Β-ΓΛΥΚΟΣΙΔΑΣΗΣ
• ΚΡΙΘΑΡΙ (HORDEUM VULGARE)
• ΤΡΑΝΣΓΑΛΑΚΤΟΣΥΛΙΩΣΗ
• ΥΔΡΟΛΥΣΗ ΛΑΚΤΟΖΗΣ
• Φυσικές Επιστήμες
• Natural Sciences
• Χημεία
• Chemical Sciences

Language dc:language

gre

Identifier

10.12681/eadd/1305

OAI identifier oai:identifier

oai:10442/1305