ΠΡΟΣΔΙΟΡΙΣΜΟΣ ΠΕΝΙΚΙΛΛΙΝΩΝ ΣΤΟ ΓΑΛΑ ΑΓΕΛΑΔΑΣ ΜΕ ΥΓΡΟΧΡΩΜΑΤΟΓΡΑΦΙΑ ΥΨΗΛΗΣ ΑΠΟΔΟΣΗΣ

A HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC (HPLC) METHOD FOR THE DETERMINATION OF PENICILLIN G, PENICILLIN V, OXACILLIN AND CLOXACILLIN IN COW'S MILK, HAS BEENDEVELOPED. PENICILLINS ARE EXTRACTED FROM MILK WITH DICHLOROMETHANE. CLEAN UP IS ACCOMPLISHED BY PARTITIONING BETWEEN BUFFERS AND ORGANIC SOLVENTS . PENICILLINS ARE FINALLY EXTRACTED INTO CHLOROFORM AS ION PAIRS WITH TETRABUTYLANNONIUM CATIONS. FOR HPLC, A REVERSED-PHASE COLUMN (NUCLEOSIL 5 C18) IS USED. THE MOBILE PHASE IS A MIXTURE OF 0,02M PHOSPHATE BUFFER AND ACETONITRILE (62:38,V/V), PH6, CONTAINING 5 MM TETRABUTYLAMMONIUM HYDROGEN SULFATE. THE ACCURACY OF THE METHOD IS 82,9+3,3% FOR PENICILLIN G, 89,3+5,1% FOR PENICILLIN V, 90,7+2% FOR OXACILLIN AND 90,6+4,6% FOR CLOXACILLIN, WHILE PRECISION DATA SHOW AN OVERALL RELATIVE STANDARD DEVIATION OF 4,61%, 6,56%, 3,77%, AND 5,83% FOR PENICILLIN G, PENICILLIN V, OXACILLIN AND CLOXACILLIN, RESPECTIVELY. THE LINEARITY OF THIS METHOD, WHICH PROVIDES QUANTITATION DOWN TO 4PPB FOR PENICILLIN G AND PENICILLIN V AND 3PPB FOR OXACILLIN AND CLOXACILLIN, IS EXCELLENT (R-2>0.998) IN THE RANGE OF 4-100PPB.

Grantor dc:publisher

Aristotle University Of Thessaloniki (AUTH)

Year dc:date

1990

Authors dc:creator

• Φλετούρης, Δημήτριος
• Fletouris, Dimitrios

• ΑΝΤΙΒΙΟΤΙΚΑ ΣΤΟ ΓΑΛΑ
• ΚΑΤΑΛΟΙΠΑ ΑΝΤΙΜΙΚΡΟΒΙΑΚΩΝ ΟΥΣΙΩΝ ΣΤΑ ΤΡΟΦΙΜΑ
• Πενικιλλίνες
• ΠΕΝΙΚΙΛΛΙΝΕΣ ΣΤΑ ΤΡΟΦΙΜΑ ΖΩΙΚΗΣ ΠΡΟΕΛΕΥΣΗΣ
• ΠΕΝΙΚΙΛΛΙΝΕΣ ΣΤΟ ΓΑΛΑ
• Υγροχρωματογραφία υψηλής απόδοσης
• ANTIBIOTICS IN MILK
• High performance liquid chromatography
• Penicillin
• PENICILLINS IN MILK
• RESIDUES OF ANTIBIOTICS IN FOOD OF ANIMAL ORIGI
• Γεωπονικές Επιστήμες και Κτηνιατρική
• Κτηνιατρική
• Agricultural and Veterinary Sciences
• Veterinary Science

Language dc:language

gre

Identifier

10.12681/eadd/1263

OAI identifier oai:identifier

oai:10442/1263