ΑΝΤΙΓΟΝΙΚΗ ΜΕΛΕΤΗ. ΤΥΠΟΠΟΙΗΣΗ ΚΑΙ ΑΝΟΣΟΕΝΖΥΜΑΤΙΚΟΣ ΕΛΕΓΧΟΣ ΕΙΔΙΚΩΝ ΑΝΤΙΣΩΜΑΤΩΝ ΕΝΑΝΤΙ ΤΩΝ ΤΥΠΩΝ 1 ΚΑΙ 2 ΤΟΥ ΙΟΥ ΤΟΥ ΑΠΛΟΥ ΕΡΠΗΤΑ (HSV)

STUDY ON THE ANTIGEN PRODUCTION OF HSV-1/733 AND HSV-2/734 FROM INFECTED CELL CULTURES, THE PRODUCTION OF MONOSPECIFIC ANTISERA, THE DETECTION AND ISOLATION OF SPECIFIC ANTIGENS FOR THE TWO TYPES OF VIRUS. TYPING OF 40 ISOLATES FROM DIFFERENT HERPETIC LESIONS OF PATIENTS. THE TYPING OF THESE ISOLATES WAS ACHIEVED BY ELISA USING THE PRODUCED MONOSPECIFIC SERA. THE RESULTS WERE CONFIRMED BY RESTRICTION ENZYME DNA ANALYSIS IN 29 CASES PROVING THAT THE MONOSPECIFIC ANTISERARAISED AGAINST THE TYPE SPECIFIC ANTIGENS OF EACH TYPE (HSV-1 OR HSV-2) CAN BEUSED IN ELISA FOR THE TYPING OF HSV ISOLATES. PRESENTATION OF RESULTS OF ANTIBODY DETECTION IN HUMAN SERA AGAINST THE TWO TYPES OF HSV. THESE RESULTS SHOWED THE PREPARED SPECIFIC ANTIGENS ARE MUCH MORE TYPE- SPECIFIC THAN AVAILABLE COMMERCIALIZED ANTIGENS AND CAN BE USED FOR THE DETECTION OF TYPE SPECIFIC ANTIBODIES BY ELISA.

Grantor dc:publisher

University of Ioannina

Year dc:date

1989

Authors dc:creator

• Markoulatos, Panagiotis
• Μαρκουλάτος, Παναγιώτης

• ELISA
• Herpes simplex virus 1
• TYPING OF ISOLATED STRAINS HSV-1, HSV-2
• Western blot
• ΑΝΟΣΟΕΝΖΥΜΑΤΙΚΟΣ ΕΛΕΓΧΟΣ ΕΙΔΙΚΩΝ ΑΝΤΙΣΩΜΑΤΩΝ
• ΑΝΤΙΓΟΝΙΚΗ ΜΕΛΕΤΗ ΤΩΝ 2 ΤΥΠΩΝ ΤΟΥ ΙΟΥ (HSV)
• ΙΟΣ ΤΟΥ ΑΠΛΟΥ ΕΡΠΗΤΑ ΤΥΠΟΥ 1 ΚΑΙ 2
• ΤΥΠΟΠΟΙΗΣΗ ΣΤΕΛΕΧΩΝ HSV-1 ΚΑΙ HSV-2
• Ιατρική και Επιστήμες Υγείας
• Κλινική Ιατρική
• Medical and Health Sciences
• Clinical Medicine

Language dc:language

gre

Identifier

10.12681/eadd/1249

OAI identifier oai:identifier

oai:10442/1249