ΙΔΙΟΤΗΤΕΣ ΤΩΝ ΘΕΣΕΩΝ ΔΕΣΜΕΥΣΗΣ L-ΓΛΟΥΤΑΜΙΚΟΥ L-ΑΣΠΑΡΤΙΚΟΥ ΚΑΙ ΚΑΙΝΙΚΟΥ ΟΞΕΟΣ ΕΓΚΕΦΑΛΟΥ ΠΤΗΝΩΝ. "ΣΥΜΒΟΛΗ ΣΤΗΝ ΑΠΟΜΟΝΩΣΗ ΚΑΙ ΧΑΡΑΚΤΗΡΙΣΜΟ ΤΩΝ ΘΕΣΕΩΝ ΔΕΣΜΕΥΣΗΣ L-ΓΛΟΥΤΑΜΙΚΟΥ ΟΞΕΟΣ"

THE BINDING KINETICS, PHARMACOLOGICAL PROPERTIES AND REGIONAL ONTOGENY OF L-GLUTAMATE, L-ASPARTATE AND KAINATE BINDING SITES HAVE BEEN STUDIED IN MEMBRANE PREPARATIONS OF CHICK BRAIN. ONE BINDING COMPONENT WAS FOUND FOR L-[3H]GLUTAMATE AND L-[3H] ASPARTATE WITH A KD VALUE OF 176 NM AND 204 RESPECTIVELY. FOR KAINIC ACID TWO BINDING COMPONENTS WERE FOUND IN THE HEMISPHERES, OPTIC LOBES AND BRAIN STEM, ONE WITH HIGH AFFINITY AND A KD VALUE OF 12.5 NM AND ONE WITH LOW AFFINITY AND A KD VALUE OF 260 NM. IN CEREBELLUM ONLY ONE BINDING SITE WAS DETECTED FOR [3H]KAINIC ACID WITH A KD VALUE OF 144 NM. L-GLUTAMATE, L-ASPARTATE, QUISQUALATE, L-HOMOCYSTEIC AND IBOTENIC ACID WERE POTENT INHIBITORS OF L-[3H] GLUTAMATE BINDING. L-ASPARTATE, L-GLUTAMATE, IBOTENATE AND QUISQUALATE WERE POTENT INHIBITORS OF L- [3H]ASPARTATE BINDING. THE ONTOGENY OF GLUTAMATE BINDING SITES WAS STUDIES IN MEMBRANE PREPARATIONS OF HEMISPHERES, OPTIC LOBES, BRAIN STEM AND CEREBELLUM. IN ALL BRAIN REGIONS STUDIED INCREASES OF SPECIFIC BINDING WERE FOUND AFTER EMBRYONIC DAY 19 AND ESPECIALLY BETWEEN DAYS 1 TO 5 OF LIFE. FOR L-ASPARTATE, IN ALL BRAIN REGIONS STUDIED MAJOR INCREASES OF BINDING WERE OBSERVED DURING THE THIRD WEEK OF THE IN OVO PERIOD OF LIFE. IN CEREBELLUM MAJOR INCREASES OF KAINIC ACID BINDING SITES APPEAR AFTER EMBRYONIC DAY 17 REACHING A PLATEAULEVEL BY POST-HATCHING DAY 5. THE DETERGENT ZWITTERGENT 3-12 WAS USED FOR SOLUBILIZATION OF L- GLUTAMATE BINDING SITES FROM CHICK BRAIN MEMBRANES CHROMATOGRAPHIC SEPARATION WITH SEPHAROSE CL-6B HAVE SHOWN THREE PICKS OF L- [3H]GLUTAMATEBINDING. THE PHOTOAFFINITY CROSS-LINKER HSAB, WAS USED TO ATTACH L-[3H]GLUTAMATE IRREVERSIBLY TO CHICK BRAIN MEMBRANES. ELECTROPHORETIC ANALYSIS WITH SDS-PAGE REVEALED A MAJOR RADIOACTIVE PROTEIN BAND WITH AN APPARENT MR OF 45600 DA. PHOTOLABELING WAS INHIBITED BY QUISQUALIC ACID.

Grantor dc:publisher

University of Patras

Year dc:date

1989

Author dc:creator

• Βουκελάτου, Γεωργία

• Binding sites
• CHICK BRAIN
• KAINATE
• L-ASPARTATE
• L-GLUTAMATE
• L-ΑΣΠΑΡΤΙΚΟ ΟΞΥ
• L-γλουταμικό οξύ
• Ontogeny
• PHARMACOLOGICAL PROPERTIES
• PHOTOLABELING
• Solubilization
• Διαλυτοποίηση
• ΕΓΚΕΦΑΛΟΣ ΚΟΤΟΠΟΥΛΟΥ
• ΘΕΣΕΙΣ ΔΕΥΣΜΕΥΣΗΣ
• Καϊνικό οξύ
• Οντογένεση
• Φαρμακολογικές ιδιότητες
• ΦΩΤΟΣΗΜΑΝΣΗ
• Ιατρική και Επιστήμες Υγείας
• Βασική Ιατρική
• Medical and Health Sciences
• Basic Medicine

Language dc:language

gre

Identifier

10.12681/eadd/1051

OAI identifier oai:identifier

oai:10442/1051