ΕΦΑΡΜΟΓΕΣ ΤΩΝ ΕΚΛΕΚΤΙΚΩΝ ΗΛΕΚΤΡΟΔΙΩΝ ΣΕ ΜΕΛΕΤΕΣ ΑΛΛΗΛΕΠΙΔΡΑΣΗΣ ΔΙΑΦΟΡΩΝ ΙΟΝΤΩΝ ΜΕ ΤΗΝ ΑΛΒΟΥΜΙΝΗ

THE MAIN SCOPE OF THIS DOCTORAL THESIS WAS TO APPLY ION- SELECTIVE ELECTRODES (ISE) TO THE STUDY OF THE BINDING OF VARIOUS IONS TO ALBUMIN. MORE SPECIFICALLY,THE FOLLOWING STUDIES WERE CARRIED OUT: 1: THE PICRATE-ALBUMIN INTERACTION WASSELECTED AS A MODEL TO STUDY THE DEPENDENCE OF THE BINDING PARAMETERS ON THE PROTEIN CONCENTRATION, THE IONIC STRENGTH, THE PH AND THE TEMPERATURE. A COMPUTER PROGRAM WAS USED TO CALCULATE THE BINDING PARAMETERS, WHICH PERFORMS NONLINEAR FIT OF THE SCATCHARD MODEL TO THE DATA. THE PROPOSED PROGRAM IS BASED ON A MODIFIED GAUSS- NEWTON- LEVENBERG-MARQUARDT ALGORITHM AND HAS THE FOLLOWING NEW FEATURES: A) THE FITTING CAN BE PERFORMED FOR ANY NUMBER OF BINDING SITES AND DIRECTLY TO THE EXPERIMENTAL DATA (ELECTRODE POTENTIAL-TOTAL LIGAND CONCENTRATION), B) IT IS POSSIBLE TO CALCULATE SIMULTANEOUSLY THE ELECTRODE PARAMETERS E' AND S, C) ORTHOGONAL TRANSFORMATIONS ARE USED INSTEAD OF THE CLASSICAL MARQUARDTALGORITHM. WITH THIS MODIFICATION THE CONVERGENCE PROCESS IS FASTER AND THE RAINDING ERRORS ARE MINIMIZED. (SHORTENED)

Grantor dc:publisher

National and Kapodistrian University of Athens

Year dc:date

1987

Authors dc:creator

• Χριστόπουλος, Θεόδωρος
• Christopoulos, Theodore

• ΑΛΛΗΛΕΠΙΔΡΑΣΗ ΠΡΩΤΕΙΝΩΝ ΜΕ ΜΙΚΡΟΜΟΡΙΑ
• ΕΚΛΕΚΤΙΚΑ ΗΛΕΚΤΡΟΔΙΑ ΙΟΝΤΩΝ
• Ποτενσιομετρία
• ΠΡΟΣΔΙΟΡΙΣΜΟΣ ΑΛΒΟΥΜΙΝΗΣ ΣΕ ΟΡΟ, ΠΛΑΣΜΑ, ΑΙΜΑ
• ASSAY OF ALBUMININ SERUM, BLOOD AND PLASMA
• ION-SELECTIVE ELECTRODES
• Potentiometry
• PROTEIN BINDING STUDIES
• Φυσικές Επιστήμες
• Χημεία
• Natural Sciences
• Chemical Sciences

Language dc:language

gre

Identifier

10.12681/eadd/0557

OAI identifier oai:identifier

oai:10442/0557