{"id":{"repo_id":"greece","oai_identifier":"oai:10442/0557"},"canonical_url":"https://search.dev.ndltd.org/etd/greece/oai:10442/0557","repository":{"repo_id":"greece","name":"Greek National Archive of PhD Theses","base_url":"https://phdtheses.ekt.gr/eadd_oai/request"},"display":{"title":"ΕΦΑΡΜΟΓΕΣ ΤΩΝ ΕΚΛΕΚΤΙΚΩΝ ΗΛΕΚΤΡΟΔΙΩΝ ΣΕ ΜΕΛΕΤΕΣ ΑΛΛΗΛΕΠΙΔΡΑΣΗΣ ΔΙΑΦΟΡΩΝ ΙΟΝΤΩΝ ΜΕ ΤΗΝ ΑΛΒΟΥΜΙΝΗ","abstract":"THE MAIN SCOPE OF THIS DOCTORAL THESIS WAS TO APPLY ION- SELECTIVE ELECTRODES (ISE) TO THE STUDY OF THE BINDING OF VARIOUS IONS TO ALBUMIN. MORE SPECIFICALLY,THE FOLLOWING STUDIES WERE CARRIED OUT: 1: THE PICRATE-ALBUMIN INTERACTION WASSELECTED AS A MODEL TO STUDY THE DEPENDENCE OF THE BINDING PARAMETERS ON THE PROTEIN CONCENTRATION, THE IONIC STRENGTH, THE PH AND THE TEMPERATURE. A COMPUTER PROGRAM WAS USED TO CALCULATE THE BINDING PARAMETERS, WHICH PERFORMS NONLINEAR FIT OF THE SCATCHARD MODEL TO THE DATA. THE PROPOSED PROGRAM IS BASED ON A MODIFIED GAUSS- NEWTON- LEVENBERG-MARQUARDT ALGORITHM AND HAS THE FOLLOWING NEW FEATURES: A) THE FITTING CAN BE PERFORMED FOR ANY NUMBER OF BINDING SITES AND DIRECTLY TO THE EXPERIMENTAL DATA (ELECTRODE POTENTIAL-TOTAL LIGAND CONCENTRATION), B) IT IS POSSIBLE TO CALCULATE SIMULTANEOUSLY THE ELECTRODE PARAMETERS E' AND S, C) ORTHOGONAL TRANSFORMATIONS ARE USED INSTEAD OF THE CLASSICAL MARQUARDTALGORITHM. WITH THIS MODIFICATION THE CONVERGENCE PROCESS IS FASTER AND THE RAINDING ERRORS ARE MINIMIZED. (SHORTENED)","abstract_html":"THE MAIN SCOPE OF THIS DOCTORAL THESIS WAS TO APPLY ION- SELECTIVE ELECTRODES (ISE) TO THE STUDY OF THE BINDING OF VARIOUS IONS TO ALBUMIN. MORE SPECIFICALLY,THE FOLLOWING STUDIES WERE CARRIED OUT: 1: THE PICRATE-ALBUMIN INTERACTION WASSELECTED AS A MODEL TO STUDY THE DEPENDENCE OF THE BINDING PARAMETERS ON THE PROTEIN CONCENTRATION, THE IONIC STRENGTH, THE PH AND THE TEMPERATURE. A COMPUTER PROGRAM WAS USED TO CALCULATE THE BINDING PARAMETERS, WHICH PERFORMS NONLINEAR FIT OF THE SCATCHARD MODEL TO THE DATA. THE PROPOSED PROGRAM IS BASED ON A MODIFIED GAUSS- NEWTON- LEVENBERG-MARQUARDT ALGORITHM AND HAS THE FOLLOWING NEW FEATURES: A) THE FITTING CAN BE PERFORMED FOR ANY NUMBER OF BINDING SITES AND DIRECTLY TO THE EXPERIMENTAL DATA (ELECTRODE POTENTIAL-TOTAL LIGAND CONCENTRATION), B) IT IS POSSIBLE TO CALCULATE SIMULTANEOUSLY THE ELECTRODE PARAMETERS E' AND S, C) ORTHOGONAL TRANSFORMATIONS ARE USED INSTEAD OF THE CLASSICAL MARQUARDTALGORITHM. WITH THIS MODIFICATION THE CONVERGENCE PROCESS IS FASTER AND THE RAINDING ERRORS ARE MINIMIZED. (SHORTENED)","abstract_has_math":false,"creators":["Χριστόπουλος, Θεόδωρος","Christopoulos, Theodore"],"institution":"National and Kapodistrian University of Athens","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1987,"date_issued":"1987","date_published":"1987","updated_at":"2026-07-24T02:25:21Z","subjects":["ΑΛΛΗΛΕΠΙΔΡΑΣΗ ΠΡΩΤΕΙΝΩΝ ΜΕ ΜΙΚΡΟΜΟΡΙΑ","ΕΚΛΕΚΤΙΚΑ ΗΛΕΚΤΡΟΔΙΑ ΙΟΝΤΩΝ","Ποτενσιομετρία","ΠΡΟΣΔΙΟΡΙΣΜΟΣ ΑΛΒΟΥΜΙΝΗΣ ΣΕ ΟΡΟ, ΠΛΑΣΜΑ, ΑΙΜΑ","ASSAY OF ALBUMININ SERUM, BLOOD AND PLASMA","ION-SELECTIVE ELECTRODES","Potentiometry","PROTEIN BINDING STUDIES","Φυσικές Επιστήμες","Χημεία","Natural Sciences","Chemical Sciences"],"languages":["gre"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["10.12681/eadd/0557"],"render_values":[{"text":"10.12681/eadd/0557","href":"https://doi.org/10.12681/eadd/0557","code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10442/hedi/0557","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Χριστόπουλος, Θεόδωρος","Christopoulos, Theodore"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["1987"]},{"key":"dc:publisher","label":"Institution","values":["National and Kapodistrian University of Athens","Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)"]},{"key":"dc:type","label":"Dc Type","values":["PhD Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["ΑΛΛΗΛΕΠΙΔΡΑΣΗ ΠΡΩΤΕΙΝΩΝ ΜΕ ΜΙΚΡΟΜΟΡΙΑ","ΕΚΛΕΚΤΙΚΑ ΗΛΕΚΤΡΟΔΙΑ ΙΟΝΤΩΝ","Ποτενσιομετρία","ΠΡΟΣΔΙΟΡΙΣΜΟΣ ΑΛΒΟΥΜΙΝΗΣ ΣΕ ΟΡΟ, ΠΛΑΣΜΑ, ΑΙΜΑ","ASSAY OF ALBUMININ SERUM, BLOOD AND PLASMA","ION-SELECTIVE ELECTRODES","Potentiometry","PROTEIN BINDING STUDIES","Φυσικές Επιστήμες","Χημεία","Natural Sciences","Chemical Sciences"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["gre"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["10.12681/eadd/0557","http://hdl.handle.net/10442/hedi/0557"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["THE MAIN SCOPE OF THIS DOCTORAL THESIS WAS TO APPLY ION- SELECTIVE ELECTRODES (ISE) TO THE STUDY OF THE BINDING OF VARIOUS IONS TO ALBUMIN. MORE SPECIFICALLY,THE FOLLOWING STUDIES WERE CARRIED OUT: 1: THE PICRATE-ALBUMIN INTERACTION WASSELECTED AS A MODEL TO STUDY THE DEPENDENCE OF THE BINDING PARAMETERS ON THE PROTEIN CONCENTRATION, THE IONIC STRENGTH, THE PH AND THE TEMPERATURE. A COMPUTER PROGRAM WAS USED TO CALCULATE THE BINDING PARAMETERS, WHICH PERFORMS NONLINEAR FIT OF THE SCATCHARD MODEL TO THE DATA. THE PROPOSED PROGRAM IS BASED ON A MODIFIED GAUSS- NEWTON- LEVENBERG-MARQUARDT ALGORITHM AND HAS THE FOLLOWING NEW FEATURES: A) THE FITTING CAN BE PERFORMED FOR ANY NUMBER OF BINDING SITES AND DIRECTLY TO THE EXPERIMENTAL DATA (ELECTRODE POTENTIAL-TOTAL LIGAND CONCENTRATION), B) IT IS POSSIBLE TO CALCULATE SIMULTANEOUSLY THE ELECTRODE PARAMETERS E' AND S, C) ORTHOGONAL TRANSFORMATIONS ARE USED INSTEAD OF THE CLASSICAL MARQUARDTALGORITHM. WITH THIS MODIFICATION THE CONVERGENCE PROCESS IS FASTER AND THE RAINDING ERRORS ARE MINIMIZED. (SHORTENED)","ΣΚΟΠΟΣ ΤΗΣ ΔΙΑΤΡΙΒΗΣ ΑΥΤΗΣ ΗΤΑΝ ΟΙ ΕΦΑΡΜΟΓΕΣ ΤΩΝ ΕΚΛΕΚΤΙΚΩΝ ΗΛΕΚΤΡΟΔΙΩΝ ΙΟΝΤΩΝ (EHI) ΣΕ ΜΕΛΕΤΕΣ ΑΛΛΗΛΕΠΙΔΡΑΣΗΣ ΔΙΑΦΟΡΩΝ ΙΟΝΤΩΝ ΜΕ ΤΗΝ ΑΛΒΟΥΜΙΝΗ. ΕΙΔΙΚΟΤΕΡΑ ΕΓΙΝΑΝ ΤΑ ΕΞΗΣ: 1. ΕΠΙΛΕΧΘΗΚΕ ΤΟ ΣΥΣΤΗΜΑ ΠΙΚΡΙΚΩΝ-ΑΛΒΟΥΜΙΝΗΣ ΩΣ ΜΟΝΤΕΛΟ ΚΑΙ ΕΓΙΝΕΕΚΤΕΤΑΜΕΝΗ ΜΕΛΕΤΗ ΤΗΣ ΕΞΑΡΤΗΣΗΣ ΤΩΝ ΠΑΡΑΜΕΤΡΩΝ ΤΗΣ ΔΕΣΜΕΥΣΗΣ ΑΠΟ ΤΗ ΣΥΓΚΕΝΤΡΩΣΗ ΤΗΣ ΠΡΩΤΕΙΝΗΣ, ΤΗΝ ΙΟΝΙΚΗ ΙΣΧΥ, ΤΟ ΡΗ ΚΑΙ ΤΗ ΘΕΡΜΟΚΡΑΣΙΑ. ΟΙ ΠΑΡΑΜΕΤΡΟΙ ΤΗΣ ΔΕΣΜΕΥΣΗΣ ΥΠΟΛΟΓΙΖΟΝΤΑΙ ΜΕ ΠΡΟΓΡΑΜΜΑ ΜΗ ΓΡΑΜΜΙΚΗΣ ΠΡΟΣΑΡΜΟΓΗΣ ΤΟΥ ΜΟΝΤΕΛΟΥ SCATCHARD ΓΙΑ ΔΥΟ ΟΜΑΔΕΣ ΘΕΣΕΩΝ. ΤΟ ΠΡΟΓΡΑΜΜΑ ΒΑΣΙΖΕΤΑΙ ΣΕ ΤΡΟΠΟΠΟΙΗΜΕΝΟ ΑΛΓΟΡΙΘΜΟ GAUSS-NEWTON- LEVENBERG-MARQUARDT ΚΑΙ ΠΑΡΟΥΣΙΑΖΕΙ ΤΑ ΕΞΗΣ ΝΕΑ ΧΑΡΑΚΤΗΡΙΣΤΙΚΑ: Α) ΠΡΟΣΑΡΜΟΖΕΤΑΙ ΤΟ ΓΕΝΙΚΕΥΜΕΝΟ ΜΟΝΤΕΛΟ SCATCHARD ΚΑΤ'ΕΥΘΕΙΑΝ ΣΤΙΣ ΠΕΙΡΑΜΑΤΙΚΕΣ ΤΙΜΕΣ, ΔΥΝΑΜΙΚΟΥ, ΟΛΙΚΗΣ ΣΥΓΚΕΝΤΡΩΣΗΣ ΤΟΥ ΙΟΝΤΟΣ, Β) ΥΠΑΡΧΕΙ Η ΔΥΝΑΤΟΤΗΤΑ ΤΗΣ ΔΕΣΜΕΥΣΗΣ, Ε' ΚΑΙ S ΤΟΥ ΗΛΕΚΤΡΟΔΙΟΥ ΜΑΖΙ ΜΕ ΤΙΣ ΠΑΡΑΜΕΤΡΟΥΣ ΤΗΣ ΔΕΣΜΕΥΣΗΣ, Γ) ΑΝΤΙ ΤΟΥ ΚΛΑΣΙΚΟΥ ΑΛΓΟΡΙΘΜΟΥ MARQUARDT ΕΦΑΡΜΟΖΟΝΤΑΙ ΟΡΘΟΓΩΝΙΟΙ ΜΕΤΑΣΧΗΜΑΤΙΣΜΟΙ ΓΙΑ ΝΑ ΕΠΙΤΑΧΥΝΘΕΙ Η ΕΠΑΝΑΛΗΠΤΙΚΗ ΔΙΑΔΙΚΑΣΙΑ ΣΥΓΚΛΙΣΕΩΣ ΚΑΙ ΝΑ ΜΕΙΩΘΟΥΝ ΤΑ ΣΦΑΛΜΑΤΑ ΤΑ ΠΡΟΕΡΧΟΜΕΝΑ ΑΠΟ ΤΟ ΣΤΡΟΓΓΥΛΕΜΑ ΤΩΝ ΑΡΙΘΜΩΝ ΚΑΤΑ ΤΟΥΣ ΥΠΟΛΟΓΙΣΜΟΥΣ. (ΠΕΡΙΚΟΠΗ)"]},{"key":"dc:title","label":"Title","values":["ΕΦΑΡΜΟΓΕΣ ΤΩΝ ΕΚΛΕΚΤΙΚΩΝ ΗΛΕΚΤΡΟΔΙΩΝ ΣΕ ΜΕΛΕΤΕΣ ΑΛΛΗΛΕΠΙΔΡΑΣΗΣ ΔΙΑΦΟΡΩΝ ΙΟΝΤΩΝ ΜΕ ΤΗΝ ΑΛΒΟΥΜΙΝΗ","APPLICATION OF ION-SELECTIVE ELECTRODES TO THE STUDY OF THE INTERACTION OF ALBUMIN WITH VARIOUS IONS"]}]}],"canonical_facts":{"dc:creator":["Χριστόπουλος, Θεόδωρος","Christopoulos, Theodore"],"dc:date":["1987"],"dc:description":["THE MAIN SCOPE OF THIS DOCTORAL THESIS WAS TO APPLY ION- SELECTIVE ELECTRODES (ISE) TO THE STUDY OF THE BINDING OF VARIOUS IONS TO ALBUMIN. MORE SPECIFICALLY,THE FOLLOWING STUDIES WERE CARRIED OUT: 1: THE PICRATE-ALBUMIN INTERACTION WASSELECTED AS A MODEL TO STUDY THE DEPENDENCE OF THE BINDING PARAMETERS ON THE PROTEIN CONCENTRATION, THE IONIC STRENGTH, THE PH AND THE TEMPERATURE. A COMPUTER PROGRAM WAS USED TO CALCULATE THE BINDING PARAMETERS, WHICH PERFORMS NONLINEAR FIT OF THE SCATCHARD MODEL TO THE DATA. THE PROPOSED PROGRAM IS BASED ON A MODIFIED GAUSS- NEWTON- LEVENBERG-MARQUARDT ALGORITHM AND HAS THE FOLLOWING NEW FEATURES: A) THE FITTING CAN BE PERFORMED FOR ANY NUMBER OF BINDING SITES AND DIRECTLY TO THE EXPERIMENTAL DATA (ELECTRODE POTENTIAL-TOTAL LIGAND CONCENTRATION), B) IT IS POSSIBLE TO CALCULATE SIMULTANEOUSLY THE ELECTRODE PARAMETERS E' AND S, C) ORTHOGONAL TRANSFORMATIONS ARE USED INSTEAD OF THE CLASSICAL MARQUARDTALGORITHM. WITH THIS MODIFICATION THE CONVERGENCE PROCESS IS FASTER AND THE RAINDING ERRORS ARE MINIMIZED. (SHORTENED)","ΣΚΟΠΟΣ ΤΗΣ ΔΙΑΤΡΙΒΗΣ ΑΥΤΗΣ ΗΤΑΝ ΟΙ ΕΦΑΡΜΟΓΕΣ ΤΩΝ ΕΚΛΕΚΤΙΚΩΝ ΗΛΕΚΤΡΟΔΙΩΝ ΙΟΝΤΩΝ (EHI) ΣΕ ΜΕΛΕΤΕΣ ΑΛΛΗΛΕΠΙΔΡΑΣΗΣ ΔΙΑΦΟΡΩΝ ΙΟΝΤΩΝ ΜΕ ΤΗΝ ΑΛΒΟΥΜΙΝΗ. ΕΙΔΙΚΟΤΕΡΑ ΕΓΙΝΑΝ ΤΑ ΕΞΗΣ: 1. ΕΠΙΛΕΧΘΗΚΕ ΤΟ ΣΥΣΤΗΜΑ ΠΙΚΡΙΚΩΝ-ΑΛΒΟΥΜΙΝΗΣ ΩΣ ΜΟΝΤΕΛΟ ΚΑΙ ΕΓΙΝΕΕΚΤΕΤΑΜΕΝΗ ΜΕΛΕΤΗ ΤΗΣ ΕΞΑΡΤΗΣΗΣ ΤΩΝ ΠΑΡΑΜΕΤΡΩΝ ΤΗΣ ΔΕΣΜΕΥΣΗΣ ΑΠΟ ΤΗ ΣΥΓΚΕΝΤΡΩΣΗ ΤΗΣ ΠΡΩΤΕΙΝΗΣ, ΤΗΝ ΙΟΝΙΚΗ ΙΣΧΥ, ΤΟ ΡΗ ΚΑΙ ΤΗ ΘΕΡΜΟΚΡΑΣΙΑ. ΟΙ ΠΑΡΑΜΕΤΡΟΙ ΤΗΣ ΔΕΣΜΕΥΣΗΣ ΥΠΟΛΟΓΙΖΟΝΤΑΙ ΜΕ ΠΡΟΓΡΑΜΜΑ ΜΗ ΓΡΑΜΜΙΚΗΣ ΠΡΟΣΑΡΜΟΓΗΣ ΤΟΥ ΜΟΝΤΕΛΟΥ SCATCHARD ΓΙΑ ΔΥΟ ΟΜΑΔΕΣ ΘΕΣΕΩΝ. ΤΟ ΠΡΟΓΡΑΜΜΑ ΒΑΣΙΖΕΤΑΙ ΣΕ ΤΡΟΠΟΠΟΙΗΜΕΝΟ ΑΛΓΟΡΙΘΜΟ GAUSS-NEWTON- LEVENBERG-MARQUARDT ΚΑΙ ΠΑΡΟΥΣΙΑΖΕΙ ΤΑ ΕΞΗΣ ΝΕΑ ΧΑΡΑΚΤΗΡΙΣΤΙΚΑ: Α) ΠΡΟΣΑΡΜΟΖΕΤΑΙ ΤΟ ΓΕΝΙΚΕΥΜΕΝΟ ΜΟΝΤΕΛΟ SCATCHARD ΚΑΤ'ΕΥΘΕΙΑΝ ΣΤΙΣ ΠΕΙΡΑΜΑΤΙΚΕΣ ΤΙΜΕΣ, ΔΥΝΑΜΙΚΟΥ, ΟΛΙΚΗΣ ΣΥΓΚΕΝΤΡΩΣΗΣ ΤΟΥ ΙΟΝΤΟΣ, Β) ΥΠΑΡΧΕΙ Η ΔΥΝΑΤΟΤΗΤΑ ΤΗΣ ΔΕΣΜΕΥΣΗΣ, Ε' ΚΑΙ S ΤΟΥ ΗΛΕΚΤΡΟΔΙΟΥ ΜΑΖΙ ΜΕ ΤΙΣ ΠΑΡΑΜΕΤΡΟΥΣ ΤΗΣ ΔΕΣΜΕΥΣΗΣ, Γ) ΑΝΤΙ ΤΟΥ ΚΛΑΣΙΚΟΥ ΑΛΓΟΡΙΘΜΟΥ MARQUARDT ΕΦΑΡΜΟΖΟΝΤΑΙ ΟΡΘΟΓΩΝΙΟΙ ΜΕΤΑΣΧΗΜΑΤΙΣΜΟΙ ΓΙΑ ΝΑ ΕΠΙΤΑΧΥΝΘΕΙ Η ΕΠΑΝΑΛΗΠΤΙΚΗ ΔΙΑΔΙΚΑΣΙΑ ΣΥΓΚΛΙΣΕΩΣ ΚΑΙ ΝΑ ΜΕΙΩΘΟΥΝ ΤΑ ΣΦΑΛΜΑΤΑ ΤΑ ΠΡΟΕΡΧΟΜΕΝΑ ΑΠΟ ΤΟ ΣΤΡΟΓΓΥΛΕΜΑ ΤΩΝ ΑΡΙΘΜΩΝ ΚΑΤΑ ΤΟΥΣ ΥΠΟΛΟΓΙΣΜΟΥΣ. (ΠΕΡΙΚΟΠΗ)"],"dc:identifier":["10.12681/eadd/0557","http://hdl.handle.net/10442/hedi/0557"],"dc:language":["gre"],"dc:publisher":["National and Kapodistrian University of Athens","Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)"],"dc:subject":["ΑΛΛΗΛΕΠΙΔΡΑΣΗ ΠΡΩΤΕΙΝΩΝ ΜΕ ΜΙΚΡΟΜΟΡΙΑ","ΕΚΛΕΚΤΙΚΑ ΗΛΕΚΤΡΟΔΙΑ ΙΟΝΤΩΝ","Ποτενσιομετρία","ΠΡΟΣΔΙΟΡΙΣΜΟΣ ΑΛΒΟΥΜΙΝΗΣ ΣΕ ΟΡΟ, ΠΛΑΣΜΑ, ΑΙΜΑ","ASSAY OF ALBUMININ SERUM, BLOOD AND PLASMA","ION-SELECTIVE ELECTRODES","Potentiometry","PROTEIN BINDING STUDIES","Φυσικές Επιστήμες","Χημεία","Natural Sciences","Chemical Sciences"],"dc:title":["ΕΦΑΡΜΟΓΕΣ ΤΩΝ ΕΚΛΕΚΤΙΚΩΝ ΗΛΕΚΤΡΟΔΙΩΝ ΣΕ ΜΕΛΕΤΕΣ ΑΛΛΗΛΕΠΙΔΡΑΣΗΣ ΔΙΑΦΟΡΩΝ ΙΟΝΤΩΝ ΜΕ ΤΗΝ ΑΛΒΟΥΜΙΝΗ","APPLICATION OF ION-SELECTIVE ELECTRODES TO THE STUDY OF THE INTERACTION OF ALBUMIN WITH VARIOUS IONS"],"dc:type":["PhD Thesis"]},"updated_at":"2026-07-24T02:25:21Z"}