ΣΥΣΤΗΜΑ ΠΡΩΤΕΙΝΟΣΥΝΘΕΣΗΣ ΕΛΕΥΘΕΡΟ ΚΥΤΤΑΡΩΝ ΑΠΟ ΣΥΚΩΤΙ ΠΟΝΤΙΚΟΥ C3HB

A CELL FREE SYSTEM OF PROTEIN SYNTHESIS OF MOUSE LIVER WAS DEVELOPED. A MOST INTERESTING FEATURE OF THIS SYSTEM IS ITS VERY HIGH ACTIVITY WITH ENDOGENOUS MRNA. A WIDE RANGE OF PRODUCTS ARE LABELLED DURING OPERATION OF THE SYSTEM. THERE IS A LAG PERIOD IN THE LABELLING OF SMALL AND HIGH MOLECULAR WEIGHT PRODUCTS. CYCLOBEXIMIDE, WHICH INHIBITS OVERALL PROTEIN SYNTHESIS BY 80% ALMOST COMPLETELY INHIBITS SYNTHESIS OF PROTEINS APPEARING LATE. OF THE POLYAMINES TESTED SPERMIDINE WEAKLY ACTIVATES AT LOW CONCENTRATIONS AND SPERMINE IS HIGHLY INHIBITORY EVEN AT LOW CONCENTRATIONS. INCREASING CONCENTRATIONS OF SPERMIDINE INTENSIFY LABELLING OF AT LEAST TWO PROTEINS WITH M.W 14.5 AND 15.5 KD. EXOGENOUS MRNA (GLOBIN MRNA, TMV RNA) CAN BE TRANSLATED IF THE SYSTEM IS TREATED EITHER BY MICROCOCCAL NUCLEASE OR BY PREINCUBATION TO EXHAUST ENDOGENOUS MRNA. IN THESECOND CASE THE PREINCUBATED SYSTEM HAS TO BE SUPPLIED WITH ADDITIONAL INITIATION FACTORS. AN INHIBITORY AGENT IS EXTRACTED WITH THE INITIATION FACTORS. THE INHIBITOR WAS PURIFIED WITH DEAE-BIOGEL A, SEPHADEX C50 CONCLUDED THAT THE RESPONSIBLE AGENT IS MOST LIKELY TRACES OF AMINOACIDS THAT DILUTE THE LABELLEDAMINOACID.

Grantor dc:publisher

Aristotle University Of Thessaloniki (AUTH)

Year dc:date

1986

Authors dc:creator

• Giannakouros, Thomas
• Γιαννακούρος, Θωμάς

• ΑΝΑΣΤΟΛΕΑΣ ΠΡΩΤΕΙΝΙΚΗΣ ΣΥΝΘΕΣΗΣ
• Βιοχημεία
• ΜΕΤΑΦΡΑΣΗ Μ-RNA
• Πολυαμίνες
• ΣΥΚΩΤΙ ΠΟΝΤΙΚΟΥ C3HB
• ΣΥΣΤΗΜΑ ΠΡΩΤΕΙΝΟΣΥΝΘΕΣΗΣ ΕΛΕΥΘΕΡΟ ΚΥΤΤΑΡ CELL FREE SYSTEM OF PROTEINSYNTHESIS
• INHIBITOR OF PROTEIN SYNTHESIS
• MOUSE {C3HB} LIVER
• Polyamines
• TRANSLATION OF Μ-RNAS
• Φυσικές Επιστήμες
• Χημεία
• Natural Sciences
• Chemical Sciences

Language dc:language

gre

Identifier

10.12681/eadd/0305

OAI identifier oai:identifier

oai:10442/0305