Comparative in vitro study of the proliferation and growth behavior of human osteoblast-like cells on various 2D-biomaterials

Bone engineering has been an important research field to enhance the utility of biomaterials for clinical bone repair, particularly through the incorporation of human osteoblast-like cells or earlier osteoprogenitor cells into a scaffold followed by the in vitro multiplication and/or differentiation of osteogenic cells before host implantation. The in vitro study of the growth behavior of osteoblasts onto the surface of the biomaterials provided a basic knowledge of cell-biomaterial interactions and did as a screening method for the development of biomaterials in vivo. In this in vitro study, human osteoblast-like cells were cultured on seven different biomaterials. The cell proliferation and cell colonization were analyzed by scanning electron microscopy and EZ4U-test. The tested biomaterials were synthetic biodegradable (MacroPore®, Ethisorb®, PDS®, Beriplast® P) and nonbiodegradable polymers (Palacos®) as well as calcium phosphate cement (BoneSource®) and titanium. Titanium and its alloys have been used worldwide in reconstructive surgery and dental implantation. In this study, we used it as a reference matrix, because their response to human osteoblast-like cells was reported in many studies. Human osteoblast-like cells cultivated on Ethisorb® showed the highest proliferation rate. The proliferation rate was statistically significant compared to Palacos®-, MacroPore®- and BoneSource®. Whereas, Beriplast®, PDS® and titanium yielded lower proliferation rates compared to the other tested biomaterials. The proliferation rates of the last-mentioned group, however, showed no statistically significant difference compared to Palacos®-, MacroPore®- and BoneSource®. SEM analysis showed no significant difference in individual cell features and cell colonization. But an infiltration and a growth of human osteoblast-like cells throughout the porous structure of Ethisorb®, which was formed by crossing fibers, was a striking different feature (microtopography). This feature can explain the high proliferation rate of Ethisorb®. The results showed that human osteoblast-like cells appear to be sensitive to substrate composition and topography.

Author dc:creator

• Itthichaisri, Chumpot

Contributors dc:contributor

• Gellrich, Nils-Claudius

• osteoblast
• biomaterial

Repository record source_url

https://freidok.uni-freiburg.de/data/1988

OAI identifier oai:identifier

oai:freidok.uni-freiburg.de:1988