{"id":{"repo_id":"freiburg-diss","oai_identifier":"oai:freidok.uni-freiburg.de:1988"},"canonical_url":"https://search.dev.ndltd.org/etd/freiburg-diss/oai:freidok.uni-freiburg.de:1988","repository":{"repo_id":"freiburg-diss","name":"University of Freiburg","base_url":"https://freidok.uni-freiburg.de/oai/oai2.php"},"display":{"title":"Comparative in vitro study of the proliferation and growth behavior of human osteoblast-like cells on various 2D-biomaterials","abstract":"Bone engineering has been an important research field to enhance the utility of biomaterials for clinical bone repair, particularly through the incorporation of human osteoblast-like cells or earlier osteoprogenitor cells into a scaffold followed by the in vitro multiplication and/or differentiation of osteogenic cells before host implantation. The in vitro study of the growth behavior of osteoblasts onto the surface of the biomaterials provided a basic knowledge of cell-biomaterial interactions and did as a screening method for the development of biomaterials in vivo. In this in vitro study, human osteoblast-like cells were cultured on seven different biomaterials. The cell proliferation and cell colonization were analyzed by scanning electron microscopy and EZ4U-test. The tested biomaterials were synthetic biodegradable (MacroPore®, Ethisorb®, PDS®, Beriplast® P) and nonbiodegradable polymers (Palacos®) as well as calcium phosphate cement (BoneSource®) and titanium. Titanium and its alloys have been used worldwide in reconstructive surgery and dental implantation. In this study, we used it as a reference matrix, because their response to human osteoblast-like cells was reported in many studies. Human osteoblast-like cells cultivated on Ethisorb® showed the highest proliferation rate. The proliferation rate was statistically significant compared to Palacos®-, MacroPore®- and BoneSource®. Whereas, Beriplast®, PDS® and titanium yielded lower proliferation rates compared to the other tested biomaterials. The proliferation rates of the last-mentioned group, however, showed no statistically significant difference compared to Palacos®-, MacroPore®- and BoneSource®. SEM analysis showed no significant difference in individual cell features and cell colonization. But an infiltration and a growth of human osteoblast-like cells throughout the porous structure of Ethisorb®, which was formed by crossing fibers, was a striking different feature (microtopography). This feature can explain the high proliferation rate of Ethisorb®. The results showed that human osteoblast-like cells appear to be sensitive to substrate composition and topography.","abstract_html":"Bone engineering has been an important research field to enhance the utility of biomaterials for clinical bone repair, particularly through the incorporation of human osteoblast-like cells or earlier osteoprogenitor cells into a scaffold followed by the in vitro multiplication and/or differentiation of osteogenic cells before host implantation. The in vitro study of the growth behavior of osteoblasts onto the surface of the biomaterials provided a basic knowledge of cell-biomaterial interactions and did as a screening method for the development of biomaterials in vivo. In this in vitro study, human osteoblast-like cells were cultured on seven different biomaterials. The cell proliferation and cell colonization were analyzed by scanning electron microscopy and EZ4U-test. The tested biomaterials were synthetic biodegradable (MacroPore®, Ethisorb®, PDS®, Beriplast® P) and nonbiodegradable polymers (Palacos®) as well as calcium phosphate cement (BoneSource®) and titanium. Titanium and its alloys have been used worldwide in reconstructive surgery and dental implantation. In this study, we used it as a reference matrix, because their response to human osteoblast-like cells was reported in many studies. Human osteoblast-like cells cultivated on Ethisorb® showed the highest proliferation rate. The proliferation rate was statistically significant compared to Palacos®-, MacroPore®- and BoneSource®. Whereas, Beriplast®, PDS® and titanium yielded lower proliferation rates compared to the other tested biomaterials. The proliferation rates of the last-mentioned group, however, showed no statistically significant difference compared to Palacos®-, MacroPore®- and BoneSource®. SEM analysis showed no significant difference in individual cell features and cell colonization. But an infiltration and a growth of human osteoblast-like cells throughout the porous structure of Ethisorb®, which was formed by crossing fibers, was a striking different feature (microtopography). This feature can explain the high proliferation rate of Ethisorb®. The results showed that human osteoblast-like cells appear to be sensitive to substrate composition and topography.","abstract_has_math":false,"creators":["Itthichaisri, Chumpot"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Gellrich, Nils-Claudius"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":null,"date_issued":"","date_published":null,"updated_at":"2026-07-24T02:22:33Z","subjects":["osteoblast","biomaterial"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://freidok.uni-freiburg.de/data/1988","outbound_label":"Repository record","outbound_source":"source_url"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Gellrich, Nils-Claudius"]},{"key":"dc:creator","label":"Author","values":["Itthichaisri, Chumpot"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:type","label":"Dc Type","values":["DoctoralThesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["osteoblast","biomaterial"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Bone engineering has been an important research field to enhance the utility of biomaterials for clinical bone repair, particularly through the incorporation of human osteoblast-like cells or earlier osteoprogenitor cells into a scaffold followed by the in vitro multiplication and/or differentiation of osteogenic cells before host implantation. The in vitro study of the growth behavior of osteoblasts onto the surface of the biomaterials provided a basic knowledge of cell-biomaterial interactions and did as a screening method for the development of biomaterials in vivo. In this in vitro study, human osteoblast-like cells were cultured on seven different biomaterials. The cell proliferation and cell colonization were analyzed by scanning electron microscopy and EZ4U-test. The tested biomaterials were synthetic biodegradable (MacroPore®, Ethisorb®, PDS®, Beriplast® P) and nonbiodegradable polymers (Palacos®) as well as calcium phosphate cement (BoneSource®) and titanium. Titanium and its alloys have been used worldwide in reconstructive surgery and dental implantation. In this study, we used it as a reference matrix, because their response to human osteoblast-like cells was reported in many studies. Human osteoblast-like cells cultivated on Ethisorb® showed the highest proliferation rate. The proliferation rate was statistically significant compared to Palacos®-, MacroPore®- and BoneSource®. Whereas, Beriplast®, PDS® and titanium yielded lower proliferation rates compared to the other tested biomaterials. The proliferation rates of the last-mentioned group, however, showed no statistically significant difference compared to Palacos®-, MacroPore®- and BoneSource®. SEM analysis showed no significant difference in individual cell features and cell colonization. But an infiltration and a growth of human osteoblast-like cells throughout the porous structure of Ethisorb®, which was formed by crossing fibers, was a striking different feature (microtopography). This feature can explain the high proliferation rate of Ethisorb®. The results showed that human osteoblast-like cells appear to be sensitive to substrate composition and topography.","Auf dem Gebiet des \"bone engineering\" werden derzeit rasante Fortschritte erzielt. Ein Schwerpunkt dient der Weiterentwicklung von Materialien für den Knochenersatz. Hierbei werden autologe Vorläuferzellen von Osteoblasten, die zuvor in vitro vermehrt wurden, in eine Trägermatrix (Scaffold) eingebracht. In dieser in vitro Studie wurden humane Osteoblasten auf sieben verschiedenen Biomaterialien kultiviert. Die Zellproliferation und Zellkolonisation wurde mittels Rasterelektronenmikroskopie (REM) und dem EZ4U-Test untersucht. Bei den Biomaterialien handelt es sich sowohl um biodegradierbare (MacroPore®, Ethisorb®, PDS®, Beriplast® P) als auch um nicht degradierbare Materialien wie Polymere (Palacos®), Calciumphosphatzemente (BoneSource®) und Metalle (Titan). Dabei wurde die höchste Proliferationsrate auf Ethisorb® beobachtet. Diese zeigte sich als signifikant höher als auf Palacos®, MacroPore® und BoneSource®. Gegenüber den anderen Materialien wiesen Beriplast®, PDS® und Titan geringe Proliferationsraten auf. Die REM-Untersuchung ergab keinen signifikanten Unterschied bezüglich Zellmerkmalen und Zellkolonisation. Im Gegensatz zu den anderen Materialien zeigten die Osteoblasten auf Ethisorb® ein dreidimensionales Wachstum innerhalb räumlicher Strukturen (Poren). Die makrotopographischen Eigenschaften von Ethisorb® könnten möglicherweise einen günstigen Effekt auf die Proliferationsrate von Osteoblasten haben. Die Ergebnisse dieser in vitro Untersuchung zeigen, dass das Wachstum von humanen Osteoblasten von den Materialeigenschaften der Trägermaterialien wie Oberflächenstruktur beeinflusst wird."]},{"key":"dc:format.medium","label":"Dc Format Medium","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Comparative in vitro study of the proliferation and growth behavior of human osteoblast-like cells on various 2D-biomaterials","Vergleichende in vitro Untersuchungen der Proliferation und des Wachstums humaner Osteoblasten auf verschiedenen 2D-Biomaterialien"]}]}],"canonical_facts":{"dc:contributor":["Gellrich, Nils-Claudius"],"dc:creator":["Itthichaisri, Chumpot"],"dc:description.abstract":["Bone engineering has been an important research field to enhance the utility of biomaterials for clinical bone repair, particularly through the incorporation of human osteoblast-like cells or earlier osteoprogenitor cells into a scaffold followed by the in vitro multiplication and/or differentiation of osteogenic cells before host implantation. The in vitro study of the growth behavior of osteoblasts onto the surface of the biomaterials provided a basic knowledge of cell-biomaterial interactions and did as a screening method for the development of biomaterials in vivo. In this in vitro study, human osteoblast-like cells were cultured on seven different biomaterials. The cell proliferation and cell colonization were analyzed by scanning electron microscopy and EZ4U-test. The tested biomaterials were synthetic biodegradable (MacroPore®, Ethisorb®, PDS®, Beriplast® P) and nonbiodegradable polymers (Palacos®) as well as calcium phosphate cement (BoneSource®) and titanium. Titanium and its alloys have been used worldwide in reconstructive surgery and dental implantation. In this study, we used it as a reference matrix, because their response to human osteoblast-like cells was reported in many studies. Human osteoblast-like cells cultivated on Ethisorb® showed the highest proliferation rate. The proliferation rate was statistically significant compared to Palacos®-, MacroPore®- and BoneSource®. Whereas, Beriplast®, PDS® and titanium yielded lower proliferation rates compared to the other tested biomaterials. The proliferation rates of the last-mentioned group, however, showed no statistically significant difference compared to Palacos®-, MacroPore®- and BoneSource®. SEM analysis showed no significant difference in individual cell features and cell colonization. But an infiltration and a growth of human osteoblast-like cells throughout the porous structure of Ethisorb®, which was formed by crossing fibers, was a striking different feature (microtopography). This feature can explain the high proliferation rate of Ethisorb®. The results showed that human osteoblast-like cells appear to be sensitive to substrate composition and topography.","Auf dem Gebiet des \"bone engineering\" werden derzeit rasante Fortschritte erzielt. Ein Schwerpunkt dient der Weiterentwicklung von Materialien für den Knochenersatz. Hierbei werden autologe Vorläuferzellen von Osteoblasten, die zuvor in vitro vermehrt wurden, in eine Trägermatrix (Scaffold) eingebracht. In dieser in vitro Studie wurden humane Osteoblasten auf sieben verschiedenen Biomaterialien kultiviert. Die Zellproliferation und Zellkolonisation wurde mittels Rasterelektronenmikroskopie (REM) und dem EZ4U-Test untersucht. Bei den Biomaterialien handelt es sich sowohl um biodegradierbare (MacroPore®, Ethisorb®, PDS®, Beriplast® P) als auch um nicht degradierbare Materialien wie Polymere (Palacos®), Calciumphosphatzemente (BoneSource®) und Metalle (Titan). Dabei wurde die höchste Proliferationsrate auf Ethisorb® beobachtet. Diese zeigte sich als signifikant höher als auf Palacos®, MacroPore® und BoneSource®. Gegenüber den anderen Materialien wiesen Beriplast®, PDS® und Titan geringe Proliferationsraten auf. Die REM-Untersuchung ergab keinen signifikanten Unterschied bezüglich Zellmerkmalen und Zellkolonisation. Im Gegensatz zu den anderen Materialien zeigten die Osteoblasten auf Ethisorb® ein dreidimensionales Wachstum innerhalb räumlicher Strukturen (Poren). Die makrotopographischen Eigenschaften von Ethisorb® könnten möglicherweise einen günstigen Effekt auf die Proliferationsrate von Osteoblasten haben. Die Ergebnisse dieser in vitro Untersuchung zeigen, dass das Wachstum von humanen Osteoblasten von den Materialeigenschaften der Trägermaterialien wie Oberflächenstruktur beeinflusst wird."],"dc:format.medium":["application/pdf"],"dc:subject":["osteoblast","biomaterial"],"dc:title":["Comparative in vitro study of the proliferation and growth behavior of human osteoblast-like cells on various 2D-biomaterials","Vergleichende in vitro Untersuchungen der Proliferation und des Wachstums humaner Osteoblasten auf verschiedenen 2D-Biomaterialien"],"dc:type":["DoctoralThesis"]},"updated_at":"2026-07-24T02:22:33Z"}