Characterization of the Genes Involved in Biosynthesis and Transport of Schizokinen, a Siderophore Produced by <em>Rhizobium leguminosarum</em> IARI 917.

<p>Iron is the 4th most abundant metal on the earth's crust and is required by most organisms as a cofactor for many enzymes; however, at physiological pH and aerobic conditions iron forms insoluble ferric oxyhydroxide polymers. Siderophores are low molecular weight compounds that scavenge ferric ions, bind with high affinity, and transport it into the cell via multicomponent transport systems. <em>Rhizobia</em> are soil dwelling organisms that form symbiotic relationships with host plants and fix atmospheric nitrogen, while the bacteria receive nutrients. <em>R. leguminosarum</em> IARI 917 produces a siderophore characterized as 'schizokinen'. In the present study, we have characterized the binding and transport kinetics of 'schizokinen' and have also attempted to identify the genes involved in its biosynthesis using mini Tn5 random mutagenesis. DNA sequence analysis of a non siderophore producing transconjugant revealed a gene involved in PAC/PAS signal transduction systems that respond to many extracellular cues.</p>

Name thesis:degree_name

MS (Master of Science)

Level thesis:degree_level

Thesis - unrestricted

Discipline thesis:degree_discipline

Biology

Year dc:date.issued

2008

Author dc:creator

• Hammond, David Jack

• Rhizobia
• siderophore
• schizokinen
• Chemical Actions and Uses
• Chemicals and Drugs
• Medicine and Health Sciences

Statement dc:rights

• Copyright by the authors.

Repository record dc:identifier

https://dc.etsu.edu/etd/2001

OAI identifier oai:identifier

oai:dc.etsu.edu:etd-3353