Studies of the Preparations and Use of Sol-Gel for Enzyme Immobilization and Analytical Applications.

<p>Much attention has been paid to immobilization of enzymes to improve enzyme stability and permit its reuse. Glucose oxidase entrapment in different kinds of silica sol-gel matrices was investigated. The enzyme showed stable activity for 11 uses in the sol-gel with tetramethyl orthosilicate (TMOS) as precursor, and at least 7 uses in the sol-gel induced electrochemically with tetraethyl orthosilicate (TEOS) as precursor. The sol-gel made with TEOS as precursor and HCl as catalyst showed stability in enzyme activity for 11 uses but the activity decreases on the same sol-gel when modified with silica nanoparticles. Factors such as optimal incubation time of glucose solution, the reproducibility between different sol-gels, and the storage time were investigated. Good linearity and analytical results on real samples were obtained. The detection method was based on a colorimetric method for determining the concentration of hydrogen peroxide produced from the oxidation reaction of glucose.</p>

Name thesis:degree_name

MS (Master of Science)

Level thesis:degree_level

Thesis - unrestricted

Discipline thesis:degree_discipline

Chemistry

Year dc:date.issued

2009

Author dc:creator

• Hamdan, Suzana

• sol-gel
• immobilization
• enzyme
• glucose oxidase
• Trinder reagent
• Analytical Chemistry
• Chemistry
• Physical Sciences and Mathematics

Statement dc:rights

• Copyright by the authors.

Repository record dc:identifier

https://dc.etsu.edu/etd/1802

OAI identifier oai:identifier

oai:dc.etsu.edu:etd-3154