Much attention has been paid to immobilization of enzymes to improve enzyme stability and permit its reuse. Glucose oxidase entrapment in different kinds of silica sol-gel matrices was investigated. The enzyme showed stable activity for 11 uses in the sol-gel with tetramethyl orthosilicate (TMOS) as precursor, and at least 7 uses in the sol-gel induced electrochemically with tetraethyl orthosilicate (TEOS) as precursor. The sol-gel made with TEOS as precursor and HCl as catalyst showed stability in enzyme activity for 11 uses but the activity decreases on the same sol-gel when modified with silica nanoparticles. Factors such as optimal incubation time of glucose solution, the reproducibility between different sol-gels, and the storage time were investigated. Good linearity and analytical results on real samples were obtained. The detection method was based on a colorimetric method for determining the concentration of hydrogen peroxide produced from the oxidation reaction of glucose.
","abstract_html":"<p>Much attention has been paid to immobilization of enzymes to improve enzyme stability and permit its reuse. Glucose oxidase entrapment in different kinds of silica sol-gel matrices was investigated. The enzyme showed stable activity for 11 uses in the sol-gel with tetramethyl orthosilicate (TMOS) as precursor, and at least 7 uses in the sol-gel induced electrochemically with tetraethyl orthosilicate (TEOS) as precursor. The sol-gel made with TEOS as precursor and HCl as catalyst showed stability in enzyme activity for 11 uses but the activity decreases on the same sol-gel when modified with silica nanoparticles. Factors such as optimal incubation time of glucose solution, the reproducibility between different sol-gels, and the storage time were investigated. Good linearity and analytical results on real samples were obtained. The detection method was based on a colorimetric method for determining the concentration of hydrogen peroxide produced from the oxidation reaction of glucose.</p>","abstract_has_math":false,"creators":["Hamdan, Suzana"],"institution":null,"degree_name":"MS (Master of Science)","degree_level":"Thesis - unrestricted","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2009,"date_issued":"2009-08-19T07:00:00Z","date_published":"2009-08-19T07:00:00Z","updated_at":"2026-07-24T02:20:55Z","subjects":["sol-gel","immobilization","enzyme","glucose oxidase","Trinder reagent","Analytical Chemistry","Chemistry","Physical Sciences and Mathematics"],"languages":[],"rights":["Copyright by the authors."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://dc.etsu.edu/etd/1802","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Hamdan, Suzana"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2009-08-19T07:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis - unrestricted"]},{"key":"thesis:degree_name","label":"Degree Name","values":["MS (Master of Science)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["sol-gel","immobilization","enzyme","glucose oxidase","Trinder reagent","Analytical Chemistry","Chemistry","Physical Sciences and Mathematics"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["Copyright by the authors."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://dc.etsu.edu/context/etd/article/3154/viewcontent/HamdanS072709f.pdf","https://dc.etsu.edu/etd/1802"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Much attention has been paid to immobilization of enzymes to improve enzyme stability and permit its reuse. Glucose oxidase entrapment in different kinds of silica sol-gel matrices was investigated. The enzyme showed stable activity for 11 uses in the sol-gel with tetramethyl orthosilicate (TMOS) as precursor, and at least 7 uses in the sol-gel induced electrochemically with tetraethyl orthosilicate (TEOS) as precursor. The sol-gel made with TEOS as precursor and HCl as catalyst showed stability in enzyme activity for 11 uses but the activity decreases on the same sol-gel when modified with silica nanoparticles. Factors such as optimal incubation time of glucose solution, the reproducibility between different sol-gels, and the storage time were investigated. Good linearity and analytical results on real samples were obtained. The detection method was based on a colorimetric method for determining the concentration of hydrogen peroxide produced from the oxidation reaction of glucose.
"]},{"key":"dc:title","label":"Title","values":["Studies of the Preparations and Use of Sol-Gel for Enzyme Immobilization and Analytical Applications."]}]}],"canonical_facts":{"dc:creator":["Hamdan, Suzana"],"dc:date.issued":["2009-08-19T07:00:00Z"],"dc:description.abstract":["Much attention has been paid to immobilization of enzymes to improve enzyme stability and permit its reuse. Glucose oxidase entrapment in different kinds of silica sol-gel matrices was investigated. The enzyme showed stable activity for 11 uses in the sol-gel with tetramethyl orthosilicate (TMOS) as precursor, and at least 7 uses in the sol-gel induced electrochemically with tetraethyl orthosilicate (TEOS) as precursor. The sol-gel made with TEOS as precursor and HCl as catalyst showed stability in enzyme activity for 11 uses but the activity decreases on the same sol-gel when modified with silica nanoparticles. Factors such as optimal incubation time of glucose solution, the reproducibility between different sol-gels, and the storage time were investigated. Good linearity and analytical results on real samples were obtained. The detection method was based on a colorimetric method for determining the concentration of hydrogen peroxide produced from the oxidation reaction of glucose.
"],"dc:identifier":["https://dc.etsu.edu/context/etd/article/3154/viewcontent/HamdanS072709f.pdf","https://dc.etsu.edu/etd/1802"],"dc:rights":["Copyright by the authors."],"dc:subject":["sol-gel","immobilization","enzyme","glucose oxidase","Trinder reagent","Analytical Chemistry","Chemistry","Physical Sciences and Mathematics"],"dc:title":["Studies of the Preparations and Use of Sol-Gel for Enzyme Immobilization and Analytical Applications."],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Thesis - unrestricted"],"thesis:degree_name":["MS (Master of Science)"]},"updated_at":"2026-07-24T02:20:55Z"}