Expression and Biochemical Function of Putative Flavonoid GT Clones from Grapefruit and Identification of New Clones using the harvEST Database.

<p>Flavonoids are plant secondary metabolites well known for many key roles in the life cycle of plants. They also can affect human health. <em>Citrus paradisi</em> is known to produce several glucosylated flavonoids and these compounds are glucosylated by enzymes known as glucosyltransferases (GTs). The focus of this research was to optimize the heterologous expression, enrichment, and biochemical characterization of grapefruit putative GT protein, PGT2, and to test the hypothesis that PGT2 is a flavonoid GT. Results showed detectable amounts of activity with quercetin, a flavonol; however, activity was lower than what would be expected if this enzyme were a flavonol-specific GT. In an additional aspect of this study, bioinformatics were used to test the hypothesis that additional putative GT clones could be identified using the harvEST database.</p>

Name thesis:degree_name

MS (Master of Science)

Level thesis:degree_level

Thesis - restricted

Discipline thesis:degree_discipline

Biology

Year dc:date.issued

2009

Author dc:creator

• Mallampalli, Venkata K. P. S

• Quercetin
• Inclusion Bodies
• Glucosyltransferase
• Citrus paradisi
• Flavonoids
• Life Sciences
• Plant Breeding and Genetics
• Plant Sciences

Statement dc:rights

• Copyright by the authors.

Repository record dc:identifier

https://dc.etsu.edu/etd/1788

OAI identifier oai:identifier

oai:dc.etsu.edu:etd-3140