Cell Toxicity and Uptake of RRR-Alpha-Tocopheryl Polyethylene Glycol 1000 Succinate (TPGS) by Various Cell ines <em>In Vitro</em>.

<p>This research focused on investigating and comparing the cytotoxicity and cellular uptake of RRR-alpha-tocopheryl polyethylene glycol succinate (TPGS, with that of alpha-tocopheryl succinate (&#945;-TS). Both TPGS and &#945;-TS are water-soluble forms of vitamin E with important clinical applications. Cytotoxicity assays with RAW 264.7 and LNCaP cells incubated overnight with TPGS or &#945;-TS at concentrations &#8805; 12.4 &#956;M suggest that &#945;-TS is more cytotoxic than TPGS. Macrophages were found to be more sensitive than LNCaP cells when treated with similar concentrations of &#945;-TS. For both cell lines, most of the TPGS or &#945;-TS taken up remained esterified after 24 hours. Our results suggest that cell death was due to TPGS and/or &#945;-TS and not alpha-tocopherol. A <em>para</em>-hydroxyanilide of &#945;-TS (<em>p</em>-HATS) that could be used to distinguish between cellular TPGS and &#945;-TS was studied. It was found that <em>p</em>-HATS can be detected electrochemically and that it is hydrolyzed to &#945;-TOH.</p>

Name thesis:degree_name

MS (Master of Science)

Level thesis:degree_level

Thesis - unrestricted

Discipline thesis:degree_discipline

Chemistry

Year dc:date.issued

2005

Author dc:creator

• Komguem Kamga, Christelle

• RAW 2647 Macrophages
• Toxicity
• Uptake
• p-HATS
• Cancer
• HPLC/ECD
• RRR-? -Tocopherol
• TPGS
• ?- tocopheryl succinate
• Chemistry
• Organic Chemistry
• Physical Sciences and Mathematics

Statement dc:rights

• Copyright by the authors.

Repository record dc:identifier

https://dc.etsu.edu/etd/1040

OAI identifier oai:identifier

oai:dc.etsu.edu:etd-2197