Determination of Lactose by Reversed-Phase High Performance Liquid Chromatography.

<p>Lactose is the common disaccharide found in dairy products. It can, however, be a source of discomfort for those whom are lactose intolerant. Therefore, it is necessary to accurately, reliably, and efficiently determine the lactose content of foods. The goal of this research was to develop a reversed-phase HPLC method with UV detection for the determination of lactose. Aminobenzoic acid was used to prepare a derivative, making detection possible in the 300-360 nm region. The stationary phase is a C8 column, with 85% methanol 15% water mixture at pH 5 as the mobile phase. A linear response of peak area to lactose concentration of 0.018 mg/mL to 0.36 mg/mL was obtained with an estimated detection limit of 0.0036 mg/mL lactose concentration. The reproducibility was established with a 4.95% relative standard deviation. The average recovery was 107%, and results were in strong agreement with the standard alkaline ferricyanide method, establishing accuracy.</p>

Name thesis:degree_name

MS (Master of Science)

Level thesis:degree_level

Thesis - unrestricted

Discipline thesis:degree_discipline

Chemistry

Year dc:date.issued

2004

Author dc:creator

• Sexton, Danessa Leann

• lactose
• carbohydrates
• HPLC
• reducing-sugars
• infant formula
• Chemistry
• Physical Sciences and Mathematics

Statement dc:rights

• Copyright by the authors.

Repository record dc:identifier

https://dc.etsu.edu/etd/878

OAI identifier oai:identifier

oai:dc.etsu.edu:etd-2035