Lactose is the common disaccharide found in dairy products. It can, however, be a source of discomfort for those whom are lactose intolerant. Therefore, it is necessary to accurately, reliably, and efficiently determine the lactose content of foods. The goal of this research was to develop a reversed-phase HPLC method with UV detection for the determination of lactose. Aminobenzoic acid was used to prepare a derivative, making detection possible in the 300-360 nm region. The stationary phase is a C8 column, with 85% methanol 15% water mixture at pH 5 as the mobile phase. A linear response of peak area to lactose concentration of 0.018 mg/mL to 0.36 mg/mL was obtained with an estimated detection limit of 0.0036 mg/mL lactose concentration. The reproducibility was established with a 4.95% relative standard deviation. The average recovery was 107%, and results were in strong agreement with the standard alkaline ferricyanide method, establishing accuracy.
","abstract_html":"<p>Lactose is the common disaccharide found in dairy products. It can, however, be a source of discomfort for those whom are lactose intolerant. Therefore, it is necessary to accurately, reliably, and efficiently determine the lactose content of foods. The goal of this research was to develop a reversed-phase HPLC method with UV detection for the determination of lactose. Aminobenzoic acid was used to prepare a derivative, making detection possible in the 300-360 nm region. The stationary phase is a C8 column, with 85% methanol 15% water mixture at pH 5 as the mobile phase. A linear response of peak area to lactose concentration of 0.018 mg/mL to 0.36 mg/mL was obtained with an estimated detection limit of 0.0036 mg/mL lactose concentration. The reproducibility was established with a 4.95% relative standard deviation. The average recovery was 107%, and results were in strong agreement with the standard alkaline ferricyanide method, establishing accuracy.</p>","abstract_has_math":false,"creators":["Sexton, Danessa Leann"],"institution":null,"degree_name":"MS (Master of Science)","degree_level":"Thesis - unrestricted","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2004,"date_issued":"2004-05-01T07:00:00Z","date_published":"2004-05-01T07:00:00Z","updated_at":"2026-07-24T02:19:35Z","subjects":["lactose","carbohydrates","HPLC","reducing-sugars","infant formula","Chemistry","Physical Sciences and Mathematics"],"languages":[],"rights":["Copyright by the authors."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://dc.etsu.edu/etd/878","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Sexton, Danessa Leann"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2004-05-01T07:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis - unrestricted"]},{"key":"thesis:degree_name","label":"Degree Name","values":["MS (Master of Science)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["lactose","carbohydrates","HPLC","reducing-sugars","infant formula","Chemistry","Physical Sciences and Mathematics"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["Copyright by the authors."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://dc.etsu.edu/context/etd/article/2035/viewcontent/SextonD041504f.pdf","https://dc.etsu.edu/etd/878"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Lactose is the common disaccharide found in dairy products. It can, however, be a source of discomfort for those whom are lactose intolerant. Therefore, it is necessary to accurately, reliably, and efficiently determine the lactose content of foods. The goal of this research was to develop a reversed-phase HPLC method with UV detection for the determination of lactose. Aminobenzoic acid was used to prepare a derivative, making detection possible in the 300-360 nm region. The stationary phase is a C8 column, with 85% methanol 15% water mixture at pH 5 as the mobile phase. A linear response of peak area to lactose concentration of 0.018 mg/mL to 0.36 mg/mL was obtained with an estimated detection limit of 0.0036 mg/mL lactose concentration. The reproducibility was established with a 4.95% relative standard deviation. The average recovery was 107%, and results were in strong agreement with the standard alkaline ferricyanide method, establishing accuracy.
"]},{"key":"dc:title","label":"Title","values":["Determination of Lactose by Reversed-Phase High Performance Liquid Chromatography."]}]}],"canonical_facts":{"dc:creator":["Sexton, Danessa Leann"],"dc:date.issued":["2004-05-01T07:00:00Z"],"dc:description.abstract":["Lactose is the common disaccharide found in dairy products. It can, however, be a source of discomfort for those whom are lactose intolerant. Therefore, it is necessary to accurately, reliably, and efficiently determine the lactose content of foods. The goal of this research was to develop a reversed-phase HPLC method with UV detection for the determination of lactose. Aminobenzoic acid was used to prepare a derivative, making detection possible in the 300-360 nm region. The stationary phase is a C8 column, with 85% methanol 15% water mixture at pH 5 as the mobile phase. A linear response of peak area to lactose concentration of 0.018 mg/mL to 0.36 mg/mL was obtained with an estimated detection limit of 0.0036 mg/mL lactose concentration. The reproducibility was established with a 4.95% relative standard deviation. The average recovery was 107%, and results were in strong agreement with the standard alkaline ferricyanide method, establishing accuracy.
"],"dc:identifier":["https://dc.etsu.edu/context/etd/article/2035/viewcontent/SextonD041504f.pdf","https://dc.etsu.edu/etd/878"],"dc:rights":["Copyright by the authors."],"dc:subject":["lactose","carbohydrates","HPLC","reducing-sugars","infant formula","Chemistry","Physical Sciences and Mathematics"],"dc:title":["Determination of Lactose by Reversed-Phase High Performance Liquid Chromatography."],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Thesis - unrestricted"],"thesis:degree_name":["MS (Master of Science)"]},"updated_at":"2026-07-24T02:19:35Z"}