Iron Acquisition in <em>Rhodococcus erythropolis</em> Strain IGTS8: Characterization of a Mutant that Does Not Produce a Siderophore.

<p>N5-59, a siderophore deficient mutant strain of <em>Rhodococcus erythropolis</em> strain IGTS8 (IGTS8) was investigated to learn more about how this poorly characterized bacterium acquires iron. N5-59 cells were starved for iron and then lysed to release any intracellular siderophore. No intracellular siderophore was detected indicating that N5-59 is not defective in the export or release of siderophore but is probably unable to synthesize siderophore. In a cross-feeding bioassay, growth of N5-59 (in an iron depleted medium) was greatly enhanced by the addition of exogenous siderophore from IGTS8 and other <em>Rhodococcus</em> species indicating that N5-59 is not defective in siderophore uptake. A DNA hybridization probe was prepared using DNA flanking the site of insertional mutation in strain N5-59. This probe was then used to clone a 6 kilobase pair, <em>Pst</em>I restriction fragment from the chromosome IGTS8. This cloned DNA is expected to contain the intact gene(s) that was interrupted in N5-59.</p>

Name thesis:degree_name

MS (Master of Science)

Level thesis:degree_level

Thesis - restricted

Discipline thesis:degree_discipline

Biology

Year dc:date.issued

2003

Author dc:creator

• Moretz, Samuel Eugene

• Iron Acquisition
• Heterobactin
• Siderophore
• Rhodococcus
• Biology
• Life Sciences

Statement dc:rights

• Copyright by the authors.

Repository record dc:identifier

https://dc.etsu.edu/etd/845

OAI identifier oai:identifier

oai:dc.etsu.edu:etd-2002