Molecular Study of Diapause via Generation of Two CDNA Libraries and Partial Cloning of the Ecdysone Receptor in <em>Sarcophaga crassipalpis</em>.

<p>The purpose of this study was to begin a study characterizing the diapause state in <em>Sarcophaga crassipalpis</em>. Recent evidence suggests the diapause syndrome is a unique developmental state with its own set of genes expressed. Two cDNA libraries from the pupal brains of diapausing and nondiapausing <em>S. crassipalpis</em> were generated. These libraries will be the basis for future work determining the expressed and repressed genes during the diapause syndrome. The genetic regulation of diapause in <em>S. crassipalpis</em> is also of interest. This species enters diapause during metamorphosis, which is under regulation of the steroid hormone 20-hydroxyecdysone (20-HE). Because the metamorphic system is suspended for diapause in this species, whether or not the EcR is expressed in the diapause state is of interest. To address this issue, a partial EcR was cloned from <em>S. crassipalpis</em> by RT-PCR. This partial EcR was found in all developmental stages tested.</p>

Name thesis:degree_name

MS (Master of Science)

Level thesis:degree_level

Thesis - restricted

Discipline thesis:degree_discipline

Biology

Year dc:date.issued

2002

Author dc:creator

• Ball, Elizabeth Nicole

• Diapause
• Ecdysone Receptor
• Sarcophaga crassipalpis
• Biology
• Life Sciences

Statement dc:rights

• Copyright by the authors.

Repository record dc:identifier

https://dc.etsu.edu/etd/663

OAI identifier oai:identifier

oai:dc.etsu.edu:etd-1820