The purpose of this study was to begin a study characterizing the diapause state in Sarcophaga crassipalpis. Recent evidence suggests the diapause syndrome is a unique developmental state with its own set of genes expressed. Two cDNA libraries from the pupal brains of diapausing and nondiapausing S. crassipalpis were generated. These libraries will be the basis for future work determining the expressed and repressed genes during the diapause syndrome. The genetic regulation of diapause in S. crassipalpis is also of interest. This species enters diapause during metamorphosis, which is under regulation of the steroid hormone 20-hydroxyecdysone (20-HE). Because the metamorphic system is suspended for diapause in this species, whether or not the EcR is expressed in the diapause state is of interest. To address this issue, a partial EcR was cloned from S. crassipalpis by RT-PCR. This partial EcR was found in all developmental stages tested.
","abstract_html":"<p>The purpose of this study was to begin a study characterizing the diapause state in <em>Sarcophaga crassipalpis</em>. Recent evidence suggests the diapause syndrome is a unique developmental state with its own set of genes expressed. Two cDNA libraries from the pupal brains of diapausing and nondiapausing <em>S. crassipalpis</em> were generated. These libraries will be the basis for future work determining the expressed and repressed genes during the diapause syndrome. The genetic regulation of diapause in <em>S. crassipalpis</em> is also of interest. This species enters diapause during metamorphosis, which is under regulation of the steroid hormone 20-hydroxyecdysone (20-HE). Because the metamorphic system is suspended for diapause in this species, whether or not the EcR is expressed in the diapause state is of interest. To address this issue, a partial EcR was cloned from <em>S. crassipalpis</em> by RT-PCR. This partial EcR was found in all developmental stages tested.</p>","abstract_has_math":false,"creators":["Ball, Elizabeth Nicole"],"institution":null,"degree_name":"MS (Master of Science)","degree_level":"Thesis - restricted","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2002,"date_issued":"2002-05-04T07:00:00Z","date_published":"2002-05-04T07:00:00Z","updated_at":"2026-07-24T02:19:07Z","subjects":["Diapause","Ecdysone Receptor","Sarcophaga crassipalpis","Biology","Life Sciences"],"languages":[],"rights":["Copyright by the authors."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://dc.etsu.edu/etd/663","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Ball, Elizabeth Nicole"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.issued","label":"Date","values":["2002-05-04T07:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis - restricted"]},{"key":"thesis:degree_name","label":"Degree Name","values":["MS (Master of Science)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Diapause","Ecdysone Receptor","Sarcophaga crassipalpis","Biology","Life Sciences"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["Copyright by the authors."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://dc.etsu.edu/context/etd/article/1820/viewcontent/balle043002.pdf","https://dc.etsu.edu/etd/663"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The purpose of this study was to begin a study characterizing the diapause state in Sarcophaga crassipalpis. Recent evidence suggests the diapause syndrome is a unique developmental state with its own set of genes expressed. Two cDNA libraries from the pupal brains of diapausing and nondiapausing S. crassipalpis were generated. These libraries will be the basis for future work determining the expressed and repressed genes during the diapause syndrome. The genetic regulation of diapause in S. crassipalpis is also of interest. This species enters diapause during metamorphosis, which is under regulation of the steroid hormone 20-hydroxyecdysone (20-HE). Because the metamorphic system is suspended for diapause in this species, whether or not the EcR is expressed in the diapause state is of interest. To address this issue, a partial EcR was cloned from S. crassipalpis by RT-PCR. This partial EcR was found in all developmental stages tested.
"]},{"key":"dc:title","label":"Title","values":["Molecular Study of Diapause via Generation of Two CDNA Libraries and Partial Cloning of the Ecdysone Receptor in Sarcophaga crassipalpis."]}]}],"canonical_facts":{"dc:creator":["Ball, Elizabeth Nicole"],"dc:date.issued":["2002-05-04T07:00:00Z"],"dc:description.abstract":["The purpose of this study was to begin a study characterizing the diapause state in Sarcophaga crassipalpis. Recent evidence suggests the diapause syndrome is a unique developmental state with its own set of genes expressed. Two cDNA libraries from the pupal brains of diapausing and nondiapausing S. crassipalpis were generated. These libraries will be the basis for future work determining the expressed and repressed genes during the diapause syndrome. The genetic regulation of diapause in S. crassipalpis is also of interest. This species enters diapause during metamorphosis, which is under regulation of the steroid hormone 20-hydroxyecdysone (20-HE). Because the metamorphic system is suspended for diapause in this species, whether or not the EcR is expressed in the diapause state is of interest. To address this issue, a partial EcR was cloned from S. crassipalpis by RT-PCR. This partial EcR was found in all developmental stages tested.
"],"dc:identifier":["https://dc.etsu.edu/context/etd/article/1820/viewcontent/balle043002.pdf","https://dc.etsu.edu/etd/663"],"dc:rights":["Copyright by the authors."],"dc:subject":["Diapause","Ecdysone Receptor","Sarcophaga crassipalpis","Biology","Life Sciences"],"dc:title":["Molecular Study of Diapause via Generation of Two CDNA Libraries and Partial Cloning of the Ecdysone Receptor in Sarcophaga crassipalpis."],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Thesis - restricted"],"thesis:degree_name":["MS (Master of Science)"]},"updated_at":"2026-07-24T02:19:07Z"}