Evaluating the role of Exo1p in chromosome breaks at FS2 in Saccharomyces cerevisiae

<p>Exo1p is one enzyme that plays a role in the repair of breaks in DNA caused by a variety of intrinsic and extrinsic factors. In this study, we hypothesized the following: (1) Lack of Exo1p will cause an increase in de novo telomere addition at DNA breaks, and (2) lack of the endonuclease Exo1p will lead to decreased fidelity of repair by homologous recombination at DNA breaks. A yeast model system was manipulated to induce replication stress, resulting in a break in DNA at fragile site FS2 on yeast chromosome III. The repair mechanism used was identified, and the resulting genome was analyzed. We did not observe increased de novo telomere formation at FS2 or decreased fidelity of repair by homologous recombination in cells lacking Exo1p. We conclude that the functional redundancy between Exo1p and other enzymes is sufficient to compensate for the loss of Exo1p function in the cell.</p>

Name thesis:degree_name

Master of Science (MS)

Level thesis:degree_level

Open Access Thesis

Discipline thesis:degree_discipline

Biology

Year dc:date.available

2020

Author dc:creator

• Autterson, Gillian

Contributors dc:contributor

• Anne Casper, Ph.D., Chair
• Aaron Liepman, Ph.D.
• Daniel Clemans, Ph.D.

• DNA Repair
• DNA Replication
• Exo1p
• S. cerevisiae
• telomere
• Yeast
• Biology

Repository record dc:identifier

https://commons.emich.edu/theses/1046

OAI identifier oai:identifier

oai:commons.emich.edu:theses-2423