Exo1p is one enzyme that plays a role in the repair of breaks in DNA caused by a variety of intrinsic and extrinsic factors. In this study, we hypothesized the following: (1) Lack of Exo1p will cause an increase in de novo telomere addition at DNA breaks, and (2) lack of the endonuclease Exo1p will lead to decreased fidelity of repair by homologous recombination at DNA breaks. A yeast model system was manipulated to induce replication stress, resulting in a break in DNA at fragile site FS2 on yeast chromosome III. The repair mechanism used was identified, and the resulting genome was analyzed. We did not observe increased de novo telomere formation at FS2 or decreased fidelity of repair by homologous recombination in cells lacking Exo1p. We conclude that the functional redundancy between Exo1p and other enzymes is sufficient to compensate for the loss of Exo1p function in the cell.
","abstract_html":"<p>Exo1p is one enzyme that plays a role in the repair of breaks in DNA caused by a variety of intrinsic and extrinsic factors. In this study, we hypothesized the following: (1) Lack of Exo1p will cause an increase in de novo telomere addition at DNA breaks, and (2) lack of the endonuclease Exo1p will lead to decreased fidelity of repair by homologous recombination at DNA breaks. A yeast model system was manipulated to induce replication stress, resulting in a break in DNA at fragile site FS2 on yeast chromosome III. The repair mechanism used was identified, and the resulting genome was analyzed. We did not observe increased de novo telomere formation at FS2 or decreased fidelity of repair by homologous recombination in cells lacking Exo1p. We conclude that the functional redundancy between Exo1p and other enzymes is sufficient to compensate for the loss of Exo1p function in the cell.</p>","abstract_has_math":false,"creators":["Autterson, Gillian"],"institution":null,"degree_name":"Master of Science (MS)","degree_level":"Open Access Thesis","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["Anne Casper, Ph.D., Chair","Aaron Liepman, Ph.D.","Daniel Clemans, Ph.D."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2020,"date_issued":"2020-01-01T08:00:00Z","date_published":"2020-01-01T08:00:00Z","updated_at":"2026-07-24T02:17:40Z","subjects":["DNA Repair","DNA Replication","Exo1p","S. cerevisiae","telomere","Yeast","Biology"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://commons.emich.edu/theses/1046","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Anne Casper, Ph.D., Chair","Aaron Liepman, Ph.D.","Daniel Clemans, Ph.D."]},{"key":"dc:creator","label":"Author","values":["Autterson, Gillian"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2021-02-16T08:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Open Access Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MS)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["DNA Repair","DNA Replication","Exo1p","S. cerevisiae","telomere","Yeast","Biology"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://commons.emich.edu/theses/1046"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Exo1p is one enzyme that plays a role in the repair of breaks in DNA caused by a variety of intrinsic and extrinsic factors. In this study, we hypothesized the following: (1) Lack of Exo1p will cause an increase in de novo telomere addition at DNA breaks, and (2) lack of the endonuclease Exo1p will lead to decreased fidelity of repair by homologous recombination at DNA breaks. A yeast model system was manipulated to induce replication stress, resulting in a break in DNA at fragile site FS2 on yeast chromosome III. The repair mechanism used was identified, and the resulting genome was analyzed. We did not observe increased de novo telomere formation at FS2 or decreased fidelity of repair by homologous recombination in cells lacking Exo1p. We conclude that the functional redundancy between Exo1p and other enzymes is sufficient to compensate for the loss of Exo1p function in the cell.
"]},{"key":"dc:title","label":"Title","values":["Evaluating the role of Exo1p in chromosome breaks at FS2 in Saccharomyces cerevisiae"]}]}],"canonical_facts":{"dc:contributor":["Anne Casper, Ph.D., Chair","Aaron Liepman, Ph.D.","Daniel Clemans, Ph.D."],"dc:creator":["Autterson, Gillian"],"dc:date.available":["2021-02-16T08:00:00Z"],"dc:description.abstract":["Exo1p is one enzyme that plays a role in the repair of breaks in DNA caused by a variety of intrinsic and extrinsic factors. In this study, we hypothesized the following: (1) Lack of Exo1p will cause an increase in de novo telomere addition at DNA breaks, and (2) lack of the endonuclease Exo1p will lead to decreased fidelity of repair by homologous recombination at DNA breaks. A yeast model system was manipulated to induce replication stress, resulting in a break in DNA at fragile site FS2 on yeast chromosome III. The repair mechanism used was identified, and the resulting genome was analyzed. We did not observe increased de novo telomere formation at FS2 or decreased fidelity of repair by homologous recombination in cells lacking Exo1p. We conclude that the functional redundancy between Exo1p and other enzymes is sufficient to compensate for the loss of Exo1p function in the cell.
"],"dc:identifier":["https://commons.emich.edu/theses/1046"],"dc:subject":["DNA Repair","DNA Replication","Exo1p","S. cerevisiae","telomere","Yeast","Biology"],"dc:title":["Evaluating the role of Exo1p in chromosome breaks at FS2 in Saccharomyces cerevisiae"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Open Access Thesis"],"thesis:degree_name":["Master of Science (MS)"]},"updated_at":"2026-07-24T02:17:40Z"}