Characterization of short chain fatty acids in microbial cultures by DART-MS and GC-MS

<p>Colonic bacterial degradation of starch and other carbon sources produces short-chain fatty acids (SCFA) such as butyrate and lactate, which are important in human health and disease. Analysis of the results of mono- and co-cultures in in vitro systems has led to the conclusion that diverse microbial organisms may each contribute in part to the complete metabolism to produce SCFA. GC- MS and DART- MS were investigated as analytical tools in the present study to evaluate the production of butyrate and lactate in a test tube and bioreactor mono- and co-cultures. The overall research goal is to investigate biological model systems and analytical methods to allow determination of lactate and butyrate levels under conditions that effectively emulate those that prevail in the gastrointestinal tract. These approaches will provide some understanding of the extent to which organisms work together to generate important biomolecules, in particular, the short-chain fatty acids.</p>

Name thesis:degree_name

Master of Science (MS)

Level thesis:degree_level

Open Access Thesis

Discipline thesis:degree_discipline

Chemistry

Year dc:date.available

2013

Author dc:creator

• Boppana, Shilpa

Contributors dc:contributor

• Steven Pernecky, Ph.D., Chair
• Ruth Ann Armitage, Ph.D.
• Deborah Heyl- Clegg, Ph.D.

• gastrointenstinal system
• organisms
• biomolecules
• Chemistry

Repository record dc:identifier

https://commons.emich.edu/theses/593

OAI identifier oai:identifier

oai:commons.emich.edu:theses-1969