Colonic bacterial degradation of starch and other carbon sources produces short-chain fatty acids (SCFA) such as butyrate and lactate, which are important in human health and disease. Analysis of the results of mono- and co-cultures in in vitro systems has led to the conclusion that diverse microbial organisms may each contribute in part to the complete metabolism to produce SCFA. GC- MS and DART- MS were investigated as analytical tools in the present study to evaluate the production of butyrate and lactate in a test tube and bioreactor mono- and co-cultures. The overall research goal is to investigate biological model systems and analytical methods to allow determination of lactate and butyrate levels under conditions that effectively emulate those that prevail in the gastrointestinal tract. These approaches will provide some understanding of the extent to which organisms work together to generate important biomolecules, in particular, the short-chain fatty acids.
","abstract_html":"<p>Colonic bacterial degradation of starch and other carbon sources produces short-chain fatty acids (SCFA) such as butyrate and lactate, which are important in human health and disease. Analysis of the results of mono- and co-cultures in in vitro systems has led to the conclusion that diverse microbial organisms may each contribute in part to the complete metabolism to produce SCFA. GC- MS and DART- MS were investigated as analytical tools in the present study to evaluate the production of butyrate and lactate in a test tube and bioreactor mono- and co-cultures. The overall research goal is to investigate biological model systems and analytical methods to allow determination of lactate and butyrate levels under conditions that effectively emulate those that prevail in the gastrointestinal tract. These approaches will provide some understanding of the extent to which organisms work together to generate important biomolecules, in particular, the short-chain fatty acids.</p>","abstract_has_math":false,"creators":["Boppana, Shilpa"],"institution":null,"degree_name":"Master of Science (MS)","degree_level":"Open Access Thesis","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["Steven Pernecky, Ph.D., Chair","Ruth Ann Armitage, Ph.D.","Deborah Heyl- Clegg, Ph.D."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013-08-01T07:00:00Z","date_published":"2013-08-01T07:00:00Z","updated_at":"2026-07-24T02:16:57Z","subjects":["gastrointenstinal system","organisms","biomolecules","Chemistry"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://commons.emich.edu/theses/593","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Steven Pernecky, Ph.D., Chair","Ruth Ann Armitage, Ph.D.","Deborah Heyl- Clegg, Ph.D."]},{"key":"dc:creator","label":"Author","values":["Boppana, Shilpa"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2014-09-30T07:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Open Access Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MS)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["gastrointenstinal system","organisms","biomolecules","Chemistry"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://commons.emich.edu/theses/593"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Colonic bacterial degradation of starch and other carbon sources produces short-chain fatty acids (SCFA) such as butyrate and lactate, which are important in human health and disease. Analysis of the results of mono- and co-cultures in in vitro systems has led to the conclusion that diverse microbial organisms may each contribute in part to the complete metabolism to produce SCFA. GC- MS and DART- MS were investigated as analytical tools in the present study to evaluate the production of butyrate and lactate in a test tube and bioreactor mono- and co-cultures. The overall research goal is to investigate biological model systems and analytical methods to allow determination of lactate and butyrate levels under conditions that effectively emulate those that prevail in the gastrointestinal tract. These approaches will provide some understanding of the extent to which organisms work together to generate important biomolecules, in particular, the short-chain fatty acids.
"]},{"key":"dc:title","label":"Title","values":["Characterization of short chain fatty acids in microbial cultures by DART-MS and GC-MS"]}]}],"canonical_facts":{"dc:contributor":["Steven Pernecky, Ph.D., Chair","Ruth Ann Armitage, Ph.D.","Deborah Heyl- Clegg, Ph.D."],"dc:creator":["Boppana, Shilpa"],"dc:date.available":["2014-09-30T07:00:00Z"],"dc:description.abstract":["Colonic bacterial degradation of starch and other carbon sources produces short-chain fatty acids (SCFA) such as butyrate and lactate, which are important in human health and disease. Analysis of the results of mono- and co-cultures in in vitro systems has led to the conclusion that diverse microbial organisms may each contribute in part to the complete metabolism to produce SCFA. GC- MS and DART- MS were investigated as analytical tools in the present study to evaluate the production of butyrate and lactate in a test tube and bioreactor mono- and co-cultures. The overall research goal is to investigate biological model systems and analytical methods to allow determination of lactate and butyrate levels under conditions that effectively emulate those that prevail in the gastrointestinal tract. These approaches will provide some understanding of the extent to which organisms work together to generate important biomolecules, in particular, the short-chain fatty acids.
"],"dc:identifier":["https://commons.emich.edu/theses/593"],"dc:subject":["gastrointenstinal system","organisms","biomolecules","Chemistry"],"dc:title":["Characterization of short chain fatty acids in microbial cultures by DART-MS and GC-MS"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Open Access Thesis"],"thesis:degree_name":["Master of Science (MS)"]},"updated_at":"2026-07-24T02:16:57Z"}