role of NF-<i>k</i>B signaling in multiple myeloma

<p>Genomic DNA was purified from Multiple Myeloma Cell Line JJN3, and BMF promoter region was PCR amplified and cloned into pCR 2.1 TOPO vector using One Shot® Chemically Competent E. coli. After confirmation of the clone through polymerase chain reaction (PCR) and restriction analysis, BMF promoter (2.5 Kb) was released from TOPO vector and subcloned into pGL3 promoter vector. The resulting pGL3-BMF-promoter vector was further amplified for MaxiPrep preparation. This plasmid can be used to study the regulation of BMF promoter by various transcription factors including NF-κB family members.</p>

Name thesis:degree_name

Master of Science (MS)

Level thesis:degree_level

Open Access Thesis

Discipline thesis:degree_discipline

Health Sciences

Year dc:date.available

2012

Author dc:creator

• Pallapati, Rachel Rajavi

Contributors dc:contributor

• SivaKumar Vallabhapurapu, PhD
• Stephen Sonstein, PhD
• Irwin Martin, PhD

• bone marrow cancer
• multiple myeloma
• NF-xB
• Health and Medical Administration

Repository record dc:identifier

https://commons.emich.edu/theses/451

OAI identifier oai:identifier

oai:commons.emich.edu:theses-1821