Genomic DNA was purified from Multiple Myeloma Cell Line JJN3, and BMF promoter region was PCR amplified and cloned into pCR 2.1 TOPO vector using One Shot® Chemically Competent E. coli. After confirmation of the clone through polymerase chain reaction (PCR) and restriction analysis, BMF promoter (2.5 Kb) was released from TOPO vector and subcloned into pGL3 promoter vector. The resulting pGL3-BMF-promoter vector was further amplified for MaxiPrep preparation. This plasmid can be used to study the regulation of BMF promoter by various transcription factors including NF-κB family members.
","abstract_html":"<p>Genomic DNA was purified from Multiple Myeloma Cell Line JJN3, and BMF promoter region was PCR amplified and cloned into pCR 2.1 TOPO vector using One Shot® Chemically Competent E. coli. After confirmation of the clone through polymerase chain reaction (PCR) and restriction analysis, BMF promoter (2.5 Kb) was released from TOPO vector and subcloned into pGL3 promoter vector. The resulting pGL3-BMF-promoter vector was further amplified for MaxiPrep preparation. This plasmid can be used to study the regulation of BMF promoter by various transcription factors including NF-κB family members.</p>","abstract_has_math":false,"creators":["Pallapati, Rachel Rajavi"],"institution":null,"degree_name":"Master of Science (MS)","degree_level":"Open Access Thesis","degree_discipline":"Health Sciences","degree_department":null,"school":null,"contributors":["SivaKumar Vallabhapurapu, PhD","Stephen Sonstein, PhD","Irwin Martin, PhD"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2012,"date_issued":"2012-08-31T07:00:00Z","date_published":"2012-08-31T07:00:00Z","updated_at":"2026-07-24T02:16:51Z","subjects":["bone marrow cancer","multiple myeloma","NF-xB","Health and Medical Administration"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://commons.emich.edu/theses/451","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["SivaKumar Vallabhapurapu, PhD","Stephen Sonstein, PhD","Irwin Martin, PhD"]},{"key":"dc:creator","label":"Author","values":["Pallapati, Rachel Rajavi"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2013-04-25T07:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Health Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Open Access Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MS)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["bone marrow cancer","multiple myeloma","NF-xB","Health and Medical Administration"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://commons.emich.edu/theses/451"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Genomic DNA was purified from Multiple Myeloma Cell Line JJN3, and BMF promoter region was PCR amplified and cloned into pCR 2.1 TOPO vector using One Shot® Chemically Competent E. coli. After confirmation of the clone through polymerase chain reaction (PCR) and restriction analysis, BMF promoter (2.5 Kb) was released from TOPO vector and subcloned into pGL3 promoter vector. The resulting pGL3-BMF-promoter vector was further amplified for MaxiPrep preparation. This plasmid can be used to study the regulation of BMF promoter by various transcription factors including NF-κB family members.
"]},{"key":"dc:title","label":"Title","values":["role of NF-kB signaling in multiple myeloma"]}]}],"canonical_facts":{"dc:contributor":["SivaKumar Vallabhapurapu, PhD","Stephen Sonstein, PhD","Irwin Martin, PhD"],"dc:creator":["Pallapati, Rachel Rajavi"],"dc:date.available":["2013-04-25T07:00:00Z"],"dc:description.abstract":["Genomic DNA was purified from Multiple Myeloma Cell Line JJN3, and BMF promoter region was PCR amplified and cloned into pCR 2.1 TOPO vector using One Shot® Chemically Competent E. coli. After confirmation of the clone through polymerase chain reaction (PCR) and restriction analysis, BMF promoter (2.5 Kb) was released from TOPO vector and subcloned into pGL3 promoter vector. The resulting pGL3-BMF-promoter vector was further amplified for MaxiPrep preparation. This plasmid can be used to study the regulation of BMF promoter by various transcription factors including NF-κB family members.
"],"dc:identifier":["https://commons.emich.edu/theses/451"],"dc:subject":["bone marrow cancer","multiple myeloma","NF-xB","Health and Medical Administration"],"dc:title":["role of NF-kB signaling in multiple myeloma"],"thesis:degree_discipline":["Health Sciences"],"thesis:degree_level":["Open Access Thesis"],"thesis:degree_name":["Master of Science (MS)"]},"updated_at":"2026-07-24T02:16:51Z"}