A comparative study of the expression of LINE, mys and SINE retrotransposon loci in somatic and germ-line tissues in peromyscus maniculatus

<p>This study was to determine if one or more loci are serving as a master gene for the amplification of LINE, ID, and <em>mys</em> elements, which mobilize via RNA, in <em>Peromyscus maniculatus</em> tissues, by the use of the RT-PCR technique. It was concluded that potentially more than one master gene is responsible for the amplification of LINE and <em>mys</em> in <em>P.maniculatus</em>. RT-PCR products of testes and brain for ID matched that of the BC1 gene, known to be the master gene for ID elements. However, none of the cloned products detected from ovary matched any gene available in the database. Therefore, L1 and ID might not be retrotransposed in the ovary, and BC1 might not be the master gene responsible for the amplification of the ID elements in ovary tissue. The ID in ovary potentially belongs to a more recent family of ID that hasn‘t been previously demonstrated in <em>P.maniculatus</em>.</p>

Name thesis:degree_name

Master of Science (MS)

Level thesis:degree_level

Open Access Thesis

Discipline thesis:degree_discipline

Biology

Year dc:date.available

2011

Author dc:creator

• Sepehri, Saedeh J.

Contributors dc:contributor

• David Kass, PhD, Chair
• Michael Angell, PhD
• Aaron Liepman, PhD

• Peromyscus maniculatus
• retrotransposons
• LINE elements
• Mys elements
• ID element
• Biology

Repository record dc:identifier

https://commons.emich.edu/theses/310

OAI identifier oai:identifier

oai:commons.emich.edu:theses-1309