DNA barcoding of Jatropha curcas

<p>"DNA barcoding involves sequencing a standard region of DNA to be used as a tool for rapid species identification. The cytochrome C oxidase gene (COXI) is widely accepted as the standard barcode for animals and currently there is no barcode for land plants. The Consortium for the Barcode of Life (CBOL) has proposed two regions of DNA to be used as a dual-locus barcode. In this study I examine the two proposed barcoding regions, ribulose-1 ,5-bisphosphate carboxylase/oxygenase (rbcL) and maturase K (matK) for their ease of amplification, discrimatory power, and sequence quality in Jatropha curcas, Jatropha podagrica, Latuca sativa, and Arabidopsis thaliana. I found that the rbcL gene in J. cureas and the 3' end of L. sativa met the three criteria for DNA barcoding. The matK gene was unamplifiable in all plants used in this study. The rbcL gene is a good candidate for use as a DNA barcode based on its discriminatory power and sequence quality while more efforts are needed to find an alternative to the matK gene"--Document.</p>

Name thesis:degree_name

Master of Science (MS) in Biology

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Biology

Year

2013

Author dc:creator

• Stephens, Terence L.

• DNA fingerprinting of plants
• Plant genetic engineering
• Jatropha--Genetics
• Biology--Classification
• Genetic markers
• Biology

Statement dc:rights

• Access is available to all users

Repository record dc:identifier

https://dc.ewu.edu/theses/173

OAI identifier oai:identifier

oai:dc.ewu.edu:theses-1172