Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 63 for “"DNA barcoding"”.
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DNA barcoding of Jatropha curcas
<p>"DNA barcoding involves sequencing a standard region of DNA to be used as a tool for rapid species identification. The cytochrome C oxidase gene (COXI) is widely accepted as the standard barcode for animals and currently there is no barcode for land plants. The Consortium for the Barcode of Life …
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DNA barcoding of forensically important flies in the Western Cape
… is damaged. Molecular identification such as DNA barcoding has therefore become a popular method of identifying species. DNA barcoding characterises species by sequencing and analysing specific regions in the genome. This technique has been used to characterise species in various countries …
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Development of Bioinformatic Tools and Procedures to Computationally Support DNA Barcoding
… investigating nucleotide alignments as part of DNA barcoding and DNA metabarcoding. In addition, the existing barcode regions were investigated to discover if possible mini-barcodes could be used in order to improve identification in a wide range of plant species. The pipelines developed in the …
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DNA barcoding e biodiversità molecolare: casi di studio nel settore ittico
… necessità. L'utilizzo di una breve sequenza di DNA per standardizzare l identificazione degli organismi ha recentemente conosciuto gli allori della cronaca sotto l intrigante termine di DNA barcoding . Un segmento di circa 650bp del gene della citocromo ossidasi I mitocondriale (COI) è stato …
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DNA Barcoding of Ayurvedic Herbal Supplements used for the Purported Treatment of COVID-19
… are consistent with the label, this study used DNA barcoding to test nine different types of herbal supplements (amla, ashwagandha, cinnamon, ginger, guduchi, tribulus, tulsi, turmeric, and vacha) associated with the Ayurvedic treatment of respiratory symptoms. A total of 54 commercial Ayurvedic …
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Applications of DNA-barcoding in the identification and understanding of grass invasions in Southern Africa
… These grasses can be difficult to identify. DNA barcoding has been suggested as an alternative method of identifying grasses in the hope of facilitating identification of existing invaders and preventing future invasions. In this study a list of all known naturalised grasses in South Africa …
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Use of PCR Cloning Combined with DNA Barcoding to Identify Fish in a Mixed-Species Product
<p>DNA barcoding is a valuable tool for fish species identification by food regulators, however, it does not perform well when multiple species are present within the same food product. PCR cloning has high potential to be used in combination with DNA barcoding to overcome this challenge. The …
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Real-Time PCR Combined with DNA Barcoding for the Authentication of Red Snapper (Lutjanus campechanus) Fillets
… as tilapia, rockfish, and other snapper species. DNA barcoding is typically used by regulatory agencies to detect seafood substitution; however, it is expensive and time-consuming. A rapid, real-time PCR assay targeting red snapper was developed previously for use in fisheries management; however, …
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DNA barcoding of forensically important blow flies (Diptera: Calliphoridae) within the Western Cape of South Africa
… step towards estimating post mortem interval. DNA barcoding can aid in the identification of unknown forensically relevant species, and this requires the comparison of DNA barcodes to reference data from known species. However, there is a lack of DNA barcode reference data of forensically …
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Use of a Novel Combination of Multiplex PCR and DNA Barcoding in Assessing Authenticity of Ginseng Products
… determine the efficacy of a novel combination of DNA barcoding and multiplex PCR to identify species in ginseng supplements. A total of 50 commercial ginseng supplements containing either Panax ginseng, Panax quinquefolius, or Panax notoginseng were obtained from an online vendor. Two composite …
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Authentication of Species in Bison Products Using Molecular Methods
… beef from domestic cattle (<em>Bos taurus</em>). DNA barcoding of the cytochrome <em>c </em>oxidase subunit 1 (CO1) gene is a commonly used method to test for meat species mislabeling. However, due to historical hybridization between the American bison and domestic cattle, additional testing is …
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Evaluating Mislabeling of Raw, Ready-To-Eat Seafood Products in Orange County, California
… to species mislabeling and substitution. DNA barcoding allows for fish species identification by extracting, amplifying, and sequencing a standardized gene target. A wide variety of fish products have been studied with DNA barcoding, but notably missing are ceviche and poke, particularly …
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An Evaluation of the Medicinal Plants of Andros Island, Bahamas
… Using the <em>rbcLmatK</em> gene coding regions, DNA barcoding can be used to identify plant species during all life stages without the need for identifying features. Previous ethnobotanical studies and surveys on Andros have never included a DNA barcoding database or an assay screening efficacy …
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Morphological, ecological and molecular examination of the seacucumber species along the Red Sea coast of Egypt and Gulf of Aqaba : with the investigation of the possibility of using DNA barcoding technique as a standard method for seacucumber ID
… of sea cucumber.The introduction of the new barcoding technique as a tool for sea cucumber identification was also tested in this study in order to assess its accuracy and potential of the technique in identifying sea cucumber individuals. A total of 18 different (species) of sea cucumber …
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Innovative molecular methods for fish species differentiation
… fish species have been developed. A DNA microarray was established using a set of 100 universal oligonucleotide probes. DNA probe signal patterns were observed to be speciesspecific, enabling species identification of unknown fish samples by comparing the generated DNA probe signal …
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Barcoding and eDNA metabarcoding of a rapidly changing Neotropical freshwater fish community
… additional methods (e.g. integrative taxonomy, DNA barcoding and other molecular diagnostic methods), associated with traditional taxonomic identification, are being increasingly implemented worldwide. Surprisingly, despite being a hugely biodiverse country, Brazil has not yet embraced these …
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Tracking Floral Visitation Using DNA Barcodes
… IPT, one promising alternative is the use of DNA barcoding. Because floral visitors eat, collect or unknowingly obtain pollen, nectar and other floral tissues, plant DNA should be recoverable from their bodies, permitting molecular identification of pollen loads. To assess the utility of plant …
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Identification of Species in Ground Meat Products Sold on the U.S. Commercial Market Using DNA-Based Methods
… both supermarkets and specialty meat retailers. DNA was extracted from each sample in duplicate and tested using DNA barcoding of the cytochrome c oxidase subunit I (COI) gene. The resulting sequences were identified at the species level using the Barcode of Life Database (BOLD). Any samples that …
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Investigation of mosquito (diptera: culicidae)-borne viruses circulating in some selected areas of Lusaka district, Zambia.
… mitochondrial cytochrome c oxidase subunit 1 DNA Barcoding. Female (2074) mosquitoes were pooled according to their genera and sampling locations. A total of 71 pools containing a maximum of 40 mosquitoes each were screened using a pan-flavivirus, pan-alphavirus, and pan-phlebovirus RT-PCR …
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