Structural Determinants for Inhibitor Recognition by the Dopamine Transporter

The dissertation will give an introduction, background and our research progress in the areas of dopamine transporter (DAT). A 3-D DAT computer model was generated in our laboratory based on the X-ray crystal structure of the bacterial leucine transporter LeuT, a distant homolog of DAT. The ability of this LeuTAa-based 3-D DAT computer model to identify specific DAT residues involved in inhibitor recognition was assessed through site-directed mutagenesis and subsequent pharmacologic characterization in the present study. Feedback from these experiments confirmed some of the suggestion from the DAT model, which is encouraging that the comparative DAT model is valid to some extent and could be further used for ligand docking and virtual screening.

Name thesis:degree_name

PhD

Level thesis:degree_level

Immediate Access

Discipline thesis:degree_discipline

Pharmacology-Toxicology

Year dc:date.available

2011

Author dc:creator

• Liu, Yi

Contributors dc:contributor

• Christopher K. Surratt
• Jeffry Madura
• Paula A. Witt-Enderby
• David A. Johnson
• Wilson S. Meng

• Dopamine transporter
• Molecular model
• Mutagenesis
• Photoaffinity labeling
• Virtual screening

Language dc:language

English

Repository record dc:identifier

https://dsc.duq.edu/etd/829

OAI identifier oai:identifier

oai:dsc.duq.edu:etd-1845