Development of Ultra-Sensitive Fluorescence Photoamplification Assays for the Detection of Molecular Recognition Events

<p>During the course of this research a novel method which couples the molecular recognition-triggered photoamplification chain in diaryl ketone adducts of dithiane with a "turn-off" or "turn-on" fluorescence-based assay for the detection of biological targets and ligands, regardless of their nature, through a molecular recognition event has been developed. This research has included several key steps, the most significant being: (1) the design of fluorophore adducts or dyads which recover fluorescence upon photocleavage for a "turn-on" assay and the identification of fluorophores which are quenched upon the photochemical release of a quencher for a "turn off" assay; (2) Optimization of the photochemistry and photoamplification of dithiane adducts of benzophenone as it applies to the development of an ultra-sensitive photoamplified fluorescence assay; (3) implementation of the method in the design and fabrication of chips for ultra-sensitive screening of microarrays, and (4) integration of this microchip assay into a fluorescence based signal transducer for ultra-sensitive detection of molecular recognition events. Proof of concept utilized biotin-avidin recognition, where avidin is coupled to the photochemical sensitizer.</p>

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Year dc:date.available

2010

Author dc:creator

• Gustafson, Tiffany Priscilla

Contributors dc:contributor

• Andrei G. Kutateladze, Ph.D.
• Joseph Angleson, Ph.D.
• Sandra S. Eaton
• Joseph Hornback
• Michelle Knowles

• Assays
• Fluorescence
• Molecular recognition
• Photoamplification
• Biology
• Molecular Biology

Statement dc:rights

• <p>Copyright is held by the author. User is responsible for all copyright compliance.</p>

Language dc:language

en

Repository record dc:identifier

https://digitalcommons.du.edu/etd/817

OAI identifier oai:identifier

oai:digitalcommons.du.edu:etd-1816