During the course of this research a novel method which couples the molecular recognition-triggered photoamplification chain in diaryl ketone adducts of dithiane with a \"turn-off\" or \"turn-on\" fluorescence-based assay for the detection of biological targets and ligands, regardless of their nature, through a molecular recognition event has been developed. This research has included several key steps, the most significant being: (1) the design of fluorophore adducts or dyads which recover fluorescence upon photocleavage for a \"turn-on\" assay and the identification of fluorophores which are quenched upon the photochemical release of a quencher for a \"turn off\" assay; (2) Optimization of the photochemistry and photoamplification of dithiane adducts of benzophenone as it applies to the development of an ultra-sensitive photoamplified fluorescence assay; (3) implementation of the method in the design and fabrication of chips for ultra-sensitive screening of microarrays, and (4) integration of this microchip assay into a fluorescence based signal transducer for ultra-sensitive detection of molecular recognition events. Proof of concept utilized biotin-avidin recognition, where avidin is coupled to the photochemical sensitizer.
","abstract_html":"<p>During the course of this research a novel method which couples the molecular recognition-triggered photoamplification chain in diaryl ketone adducts of dithiane with a "turn-off" or "turn-on" fluorescence-based assay for the detection of biological targets and ligands, regardless of their nature, through a molecular recognition event has been developed. This research has included several key steps, the most significant being: (1) the design of fluorophore adducts or dyads which recover fluorescence upon photocleavage for a "turn-on" assay and the identification of fluorophores which are quenched upon the photochemical release of a quencher for a "turn off" assay; (2) Optimization of the photochemistry and photoamplification of dithiane adducts of benzophenone as it applies to the development of an ultra-sensitive photoamplified fluorescence assay; (3) implementation of the method in the design and fabrication of chips for ultra-sensitive screening of microarrays, and (4) integration of this microchip assay into a fluorescence based signal transducer for ultra-sensitive detection of molecular recognition events. Proof of concept utilized biotin-avidin recognition, where avidin is coupled to the photochemical sensitizer.</p>","abstract_has_math":false,"creators":["Gustafson, Tiffany Priscilla"],"institution":null,"degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":null,"degree_department":null,"school":null,"contributors":["Andrei G. Kutateladze, Ph.D.","Joseph Angleson, Ph.D.","Sandra S. Eaton","Joseph Hornback","Michelle Knowles"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2010,"date_issued":"2010-01-01T08:00:00Z","date_published":"2010-01-01T08:00:00Z","updated_at":"2026-07-24T02:02:30Z","subjects":["Assays","Fluorescence","Molecular recognition","Photoamplification","Biology","Molecular Biology"],"languages":["en"],"rights":["Copyright is held by the author. User is responsible for all copyright compliance.
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"],"dc:format":["application/pdf"],"dc:identifier":["https://digitalcommons.du.edu/etd/817"],"dc:language":["en"],"dc:rights":["Copyright is held by the author. User is responsible for all copyright compliance.
"],"dc:subject":["Assays","Fluorescence","Molecular recognition","Photoamplification","Biology","Molecular Biology"],"dc:title":["Development of Ultra-Sensitive Fluorescence Photoamplification Assays for the Detection of Molecular Recognition Events"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."]},"updated_at":"2026-07-24T02:02:30Z"}