Regulation of Actin Dynamics During Drosophila Germband Extension

<p>Convergent extension is a process that occurs in the development of a wide variety of organisms, including gastrulation in the <em>Drosophila</em> embryo to begin to lay out the adult body plan. In fly embryos, this is known as germband extension and is mainly driven by cell intercalation or neighbor exchange by planar polarized cell-cell interface contraction to shorten the tissue along the dorsal-ventral axis. In this thesis, I show that interface contraction consists of phases of fast interface shortening and intervals of stable interface size. My data also suggests that regulation of F-actin aggregates at these shrinking interfaces is important for appropriate biphasic interface contraction. Knock down of two F-actin regulating proteins, Dpod1 and coronin, results in aberrant interface dynamics and severe disruption of germband extension. Close examination of interface and F-actin aggregate dynamics in coronin knock down reveals that coronin is required for appropriate F-actin globule formation and interface contraction.</p>

Name thesis:degree_name

M.S.

Level thesis:degree_level

Masters Thesis

Year dc:date.available

2013

Author dc:creator

• Motlong, Ashley M.

Contributors dc:contributor

• James T. Blankenship, Ph.D.
• Dinah Loerke
• Scott Barbee

• Drosophila
• Convergent extension
• F-actin
• Biochemistry, Biophysics, and Structural Biology
• Life Sciences
• Other Biochemistry, Biophysics, and Structural Biology

Statement dc:rights

• <p>Copyright is held by the author. User is responsible for all copyright compliance.</p>

Language dc:language

en

Repository record dc:identifier

https://digitalcommons.du.edu/etd/457

OAI identifier oai:identifier

oai:digitalcommons.du.edu:etd-1456