Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 152 for “"F-actin"”.
-
Mechanical properties of F-actin network
… surroundings through cytoskeletal dynamics. Actin, the most abundant protein found in eukaryotic cells, is organized into various cytoskeletal structures that provide physical support for the cell and play important roles in numerous cellular processes. Assembly of F-actin into higher-order …
-
Direct Redox Regulation of F-Actin Assembly and Disassembly by MICAL
… exert their precise effects on the internal actin cytoskeleton is poorly understood. Such effects are critical for diverse cellular behaviors including polarity, morphology, adhesion, motility, process elongation, navigation, and connectivity. Semaphorins, for example, are one of the largest …
-
Direct Redox Regulation of F-Actin Assembly and Disassembly by MICAL
… exert their precise effects on the internal actin cytoskeleton is poorly understood. Such effects are critical for diverse cellular behaviors including polarity, morphology, adhesion, motility, process elongation, navigation, and connectivity. Semaphorins, for example, are one of the largest …
-
Regulation of Mical Redox Post-Translationally-Driven F-Actin Cytoskeletal Dynamics
The actin cytoskeleton is critical for multiple diverse cellular behaviors, including the ability of an axon to form, extend, navigate, and synapse with its target. Therefore, an important goal is to understand the mechanisms that regulate it. We have been studying one of the largest families of …
-
Structural Mechanisms of F-actin Mechanochemical Regulation and Flexible Network Assembly
<p>The actin cytoskeleton integrates physical and chemical cues from a cell's environment to instruct its behavior, coordinating processes including cell migration, organelle dynamics, and endocytosis. Actin-binding proteins (ABPs) specify actin filament (F-actin) organization to build micron-scale …
-
Ca²⁺ and phosphoinositides regulations in α-actinin -4 F-actin binding.
α-actinin-4 is a non-muscle isoform of α-actinin that belongs to the spectrin superfamily. It comprises three functional regions: an N-terminal actin-binding region that consists of two calponin homology (CH) domains, a central region that consists of four copies of the spectrin-like repeat domain …
-
Functional and structural studies of a small F-actin binding domain
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1992.
-
Disrupting dynamic f-actin enhances skeletal muscle contraction due to mechanical softening
… of skeletal muscle by targeting the dynamic f-actin cytoskeleton. Using two model systems, an ex-vivo mouse muscle model and a novel in vitro optogenetic skeletal muscle micro-tissue model, we show that disruption of the dynamic f-actin cytoskeleton using small molecule actin dynamics …
-
Bitesize bundles F-actin and influences actin remodeling in syncytial Drosophila embryo development
Actin networks undergo rearrangements that influence cell and tissue shape. Actin assembly and organization is regulated in space and time by a host of actin binding proteins. Despite the importance of these processes, their regulation is not well understood. The Drosophila Synaptotagmin-like …
-
A Study of the Effects of Polyethylene Glycol on Interactions Between Glycolytic Enzymes and F-Actin, and Among Glycolytic Enzymes
… (PFK, EC 2.7.1.11) and with F-actin have been studied in the presence of PEG. Rabbit muscle glycolytic enzymes, either purified or as in myogen, were centrifuged in the presence or absence of F-actin and/or PEG and/or KCl for 35 minutes at 145,000 x g. The supernate and pellet …
-
Structual Visualization of Cytoskeletal Force Tranduction
… mechanically interfacing their intracellular actin cytoskeletons with their local extracellular environments. This enables contractile forces generated by myosin motors to mediate transduction of mechanical cues into biochemical signaling pathways by unclear mechanisms. In this thesis, I show …
-
Regulation of Actin Dynamics During Drosophila Germband Extension
… size. My data also suggests that regulation of F-actin aggregates at these shrinking interfaces is important for appropriate biphasic interface contraction. Knock down of two F-actin regulating proteins, Dpod1 and coronin, results in aberrant interface dynamics and severe disruption of germband …
-
Characterizing New Molecules and Mechanisms of Semaphorin/Plexin/MICAL Signaling
… to exert precise effects on the cell’s internal actin cytoskeleton. To help characterize an underlying logic for these cellular changes and proper cellular function I have been characterizing how one of the largest families of extracellular guidance cues, members of the Semaphorin family of …
-
The Interaction of Cofilin with the Actin Filament
The regulation of filamentous actin: F-actin) production from the polymerization of globular actin: G-actin) within the cell is critical for many cell functions. Since actin is found in all cells, understanding how actin-binding-proteins: ABPs) bind and how their regulating mechanisms work is not …
-
Calcium, actin and the spectrin skeleton: Responses to swelling and shrinking perturbations.
… incubated in cytochalasin, a drug that promotes actin depolymerization, do not undergo VLD recovery. Microscopy studies into how neuronal F-actin responded to osmotic perturbations and how the F-actin is related to VLDs were initiated. In Lymnaea neurons that had swelled then reshrunk to form …
-
Regulation and Targeting of Fyn in Human Cutaneous Squamous Cell Carcinoma
… a rapid (5-60 min.) increase in the cortical F-actin cytoskeleton. Treatment of the HaCaT-Fyn cells with Dasatinib also resulted in upregulation of F-actin. Induced F-actin colocalized with the adherens junction proteins α-catenin, β-catenin and p120catenin in HaCaT-Ras cells, suggesting that …
-
Insights into protein function from evolutionary and conformational dynamics
… in a class of proteins that bundle filamentous actin (F-actin)?" Through simulation we demonstrate that cooperative F-actin bundling tends to strengthen bundles by driving the formation of cross-links between neighboring filaments while depleting F-actin binding sites that are occupied but not …
-
Contribution of troponin-I to the regulation of cardiac muscle
… it has been possible to investigate the F-actin binding capability of the N-terminal and C-terminal regions of cTnI. The extreme C-terminal region of (human) hcTnI was demonstrated to interact with F-actin and assist in the localisation of hcTnI to the thin filament. This thesis also …
-
Opposing Actin Networks Modulate the Mechano-Activation of the Integrin LFA-1 During Immunological Synapse formation
… to APCs there is a robust accumulation of F-actin on the T cell side of the synapse. This F-actin response is characterized by robust polymerization at the periphery of the contact site followed by centripetal flow of the network towards the center of the IS. While it is well known that this …
Page 1 of 8