Determination of fatty acid composition of oils by mass spectrometry

The aim of my thesis was the determination of fatty acid content in the oils using mass spectrometry. I investigated the distribution of the fatty acid composition in beard oil, coconut oil, fish oil, palm oil and sunflower oil using GC-MS. Saponification was used as the first step in sample preparation and then I prepared the free fatty acid. HCl and Methanol is added to the sample to produce the FAMEs (Fatty acid methyl esters) done by acid catalyzed process. After this I identified these fatty acids by comparing their retention times to the FAMEs standard. Also using the chromatogram obtained from this experiment I could compare the compounds with the similarity search in the mass spectra library. Based on this I identified the various fatty acid compounds and determine the name.

Department dc:contributor.department

DE--Természettudományi és Technológiai Kar--Kémiai Intézet

Author dc:creator

• Fasasi, Gideon Ifeoluwa

Advisor dc:contributor.advisor

• Nagy, Tibor

• Fatty acid
• GC-MS
• Methylation

Language dc:language.iso

en

Handle dc:identifier.uri

http://hdl.handle.net/2437/245775

OAI identifier oai:identifier

oai:dea.lib.unideb.hu:2437/245775