The Graduate School and University Center of The City University of New York
Eukaryotic Initiation Factor 3 Interactions with Structural Elements of Barley Yellow Dwarf Virus Untranslated Regions Reveal Details of a New Cap-Independent Translation Initiation Model
Abstract
dc:description.abstract<p>Barley Yellow Dwarf Virus (BYDV) is a positive strand RNA plant virus that translates without using a 5′ 7-methylguanosine cap or a 3′ poly-adenosine tail, features that are required for canonical mRNA translation. BYDV’s non-canonical translation relies on RNA structures in the 5′ and 3′ untranslated regions (UTRs) to recruit eukaryotic initiation factors (eIFs) and ribosomes. BYDV’s 3′ translation enhancer (BTE) is a cruciform structure capable of recruiting the cap-binding complex eIF4F, the large scaffolding complex eIF3, and the 40S ribosomal subunit. Together eIF3, eIF4F, and BTE influence factor binding and 40S recruitment on the 5′ UTR and play a key role in facilitating BYDV translation. This report focuses on eIF3 by providing and analyzing data that suggest novel eIF3 functionality and that expand upon the previous model of BYDV translation initiation to include eIF3 interactions with the UTRs and other factors. Specifically, we show that eIF3 can act as a dynamic, eIF4F-responsive bridge between specific loops found in both of BYDV’s UTRs. This report also provides insight into some of the broader applications of these discoveries by highlighting both parallels and distinctions between eIF-RNA interactions of BYDV and similar interactions found in other, non-canonically translating viral mRNAs and cellular mRNAs.</p>
Degree
thesis:*- Name thesis:degree_name
- Doctor of Philosophy
- Level thesis:degree_level
- Doctoral
- Discipline thesis:degree_discipline
- Chemistry
- Grantor
- The Graduate School and University Center of The City University of New York
- Year dc:date.available
- 2022
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Powell, Paul S
- Advisor dc:contributor.advisor
-
- Dixie J Goss
- Committee members dc:contributor.committeemember
-
- Ruben Gonzalez
- Frida Kleiman
- David Jeruzalmi
Subjects
dc:subject × 5Identifiers
dc:identifier.*- Repository record dc:identifier
- https://academicworks.cuny.edu/gc_etds/4623
- OAI identifier oai:identifier
- oai:academicworks.cuny.edu:gc_etds-5702