<i>Dictyostelium discoideum</i> Protein Kinase C-orthologue <i>pkcA</i> Regulates the Actin Cytoskeleton through Interaction with Phospholipase D and p21-activated Kinase

<p>Proper regulation of the actin cytoskeleton is crucial to many cellular processes. Many of these processes are regulated by extracellular signaling cues, which direct changes in the actin cytoskeleton, resulting in changes to cellular morphology, and directed motility. The social amoeba, Dictyostelium discoideum, is used as a simple model system to study the translation of extracellular signals to the actin cytoskeleton. When starved, these unicellular amoebae undergo a multicellular developmental process characterized by a tightly regulated sequence of signaling events. This results in chemotaxis and formation of a multicellular aggregate, and ultimately cell differentiation and the formation of a fruiting body. pkcA, a putative Protein Kinase C-orthologue, has previously been shown to regulate development and fruiting body formation. Here we show that pkcA is involved in cell density sensing, chemotactic signaling, cell morphology, and cell-cell cohesion. Additionally, we show through epistasis analysis, that pkcA regulates these processes through a functional interaction with pldB, a D. discoideum PLD-orthologue. Lastly, we provide evidence that pakD, a p21-activated kinase, acts between pkcA and pldB in the regulation of development. Given that both pldB and pakD have been shown to regulate the actin cytoskeleton during chemotaxis and development, this provides a mechanism for pkcA to influence the organization and assembly of the actin cytoskeleton.</p>

Name thesis:degree_name

Doctor of Philosophy

Level thesis:degree_level

Doctoral

Discipline thesis:degree_discipline

Biology

Grantor

The Graduate School and University Center of The City University of New York

Year dc:date.available

2020

Author dc:creator

• Singh, Sean

Advisor dc:contributor.advisor

• Derrick T. Brazill

Committee members dc:contributor.committeemember

• Patricia Rockwell
• Peter Lipke
• David Foster
• Jeffrey E. Segall

• Cell Biology
• Developmental Biology
• Molecular Biology
• Dictyostelium discoideum
• cell signaling
• actin cytoskeleton
• Protein Kinase C
• Phospholipase D
• p21-activated kinase

Repository record dc:identifier

https://academicworks.cuny.edu/gc_etds/3867

OAI identifier oai:identifier

oai:academicworks.cuny.edu:gc_etds-4839